Dirofilaria repens microfilariae in Aedes vexans mosquitoes in
Slovakia

J 2013

Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia

BOCKOVÁ, Eva, Ivo RUDOLF, Alica KOČIŠOVÁ, Lenka BETÁŠOVÁ, Kristýna VENCLÍKOVÁ et. al.

Basic information

Original name

Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia

Authors

BOCKOVÁ, Eva (703 Slovakia, guarantor), Ivo RUDOLF (203 Czech Republic, belonging to the institution), Alica KOČIŠOVÁ (703 Slovakia), Lenka BETÁŠOVÁ (703 Slovakia), Kristýna VENCLÍKOVÁ (203 Czech Republic, belonging to the institution), Jan MENDEL (203 Czech Republic) and Zdeněk HUBÁLEK (203 Czech Republic, belonging to the institution)

Edition

Parasitology Research, 2013, 0932-0113

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.327

RIV identification code

RIV/00216224:14310/13:00069247

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00436-013-3526-9

UT WoS

000324326500010

Keywords in English

Aedes vexans; microfilariae; Dirofilaria repens; Dirofilaria immitis; canine dirofilariosis; Slovakia

Abstract

V originále

t In this study, we screened field-caught mosquitoes for presence of Dirofilaria spp. by using a polymerase chain reaction (PCR) assay. Potential occurrence of Dirofilaria repens and Dirofilaria immitis microfilariae was examined in 3,600 mosquitoes of eight species (Aedes vexans, Aedes cinereus, Aedes rossicus, Culex pipiens, Culiseta annulata, Ochlerotatus sticticus, Ochlerotatus cantans and Ochlerotatus caspius) collected from five locations in two districts (Kosice and Trebisov) of Eastern Slovakia, endemic region of canine dirofilariasis. Collection of mosquitoes was performed between May and August 2012 in premises known to be inhabited by Dirofilaria-infected dogs. PCR assays were performed on 72 pools, each pool containing 50 mosquitoes of the same species, collected on the same location. Each pool was examined separately for the presence of D. immitis and D. repens, respectively. A positive finding ofD. repenswas recorded in one pool of A. vexans mosquitoes collected in Košické Olšany village. Minimum infection rate in A. vexans was 1:1,750, i.e. 0.57 per 1,000 mosquitoes. The identity of D. repens was confirmed by direct sequencing of PCR product which has shown 100 % homology with sequence attributed to D. repens (GenBank accession number AJ271614). This study represents the first molecular evidence of D. repensmicrofilariae in mosquitoes in Slovakia and highlights a need for better surveillance of zoonotic dirofilariasis in central Europe.

Citovat

BOCKOVÁ, Eva, Ivo RUDOLF, Alica KOČIŠOVÁ, Lenka BETÁŠOVÁ, Kristýna VENCLÍKOVÁ, Jan MENDEL and Zdeněk HUBÁLEK. Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia. Parasitology Research. 2013, vol. 112, No 10, p. 3465-3470. ISSN 0932-0113. Available from: https://dx.doi.org/10.1007/s00436-013-3526-9.

@article{1121893,
   author = {Bocková, Eva and Rudolf, Ivo and Kočišová, Alica and Betášová, Lenka and Venclíková, Kristýna and Mendel, Jan and Hubálek, Zdeněk},
   article_number = {10},
   doi = {http://dx.doi.org/10.1007/s00436-013-3526-9},
   keywords = {Aedes vexans; microfilariae; Dirofilaria repens; Dirofilaria immitis; canine dirofilariosis; Slovakia},
   language = {eng},
   issn = {0932-0113},
   journal = {Parasitology Research},
   title = {Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia},
   volume = {112},
   year = {2013}
}

TY  - JOUR
ID  - 1121893
AU  - Bocková, Eva - Rudolf, Ivo - Kočišová, Alica - Betášová, Lenka - Venclíková, Kristýna - Mendel, Jan - Hubálek, Zdeněk
PY  - 2013
TI  - Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia
JF  - Parasitology Research
VL  - 112
IS  - 10
SP  - 3465-3470
EP  - 3465-3470
SN  - 09320113
KW  - Aedes vexans
KW  - microfilariae
KW  - Dirofilaria repens
KW  - Dirofilaria immitis
KW  - canine dirofilariosis
KW  - Slovakia
N2  - t In this study, we screened field-caught mosquitoes for presence of Dirofilaria spp. by using a polymerase chain reaction (PCR) assay. Potential occurrence of Dirofilaria repens and Dirofilaria immitis microfilariae was examined in 3,600 mosquitoes of eight species (Aedes vexans, Aedes cinereus, Aedes rossicus, Culex pipiens, Culiseta annulata, Ochlerotatus sticticus, Ochlerotatus cantans and Ochlerotatus caspius) collected from five locations in two districts (Kosice and Trebisov) of Eastern Slovakia, endemic region of canine dirofilariasis. Collection of mosquitoes was performed between May and August 2012 in premises known to be inhabited by Dirofilaria-infected dogs. PCR assays were performed on 72 pools, each pool containing 50 mosquitoes of the same species, collected on the same location. Each pool was examined separately for the presence of D. immitis and D. repens, respectively. A positive finding ofD. repenswas recorded in one pool of A. vexans mosquitoes collected in Košické Olšany village. Minimum infection rate in A. vexans was 1:1,750, i.e. 0.57 per 1,000 mosquitoes. The identity of D. repens was confirmed by direct sequencing of PCR product which has shown 100 % homology with sequence attributed to D. repens (GenBank accession number AJ271614). This study represents the first molecular evidence of D. repensmicrofilariae in mosquitoes in Slovakia and highlights a need for better surveillance of zoonotic dirofilariasis in central Europe.
ER  -

BOCKOVÁ, Eva, Ivo RUDOLF, Alica KOČIŠOVÁ, Lenka BETÁŠOVÁ, Kristýna VENCLÍKOVÁ, Jan MENDEL and Zdeněk HUBÁLEK. Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia. \textit{Parasitology Research}. 2013, vol.~112, No~10, p.~3465-3470. ISSN~0932-0113. Available from: https://dx.doi.org/10.1007/s00436-013-3526-9.

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