Bilateral Changes of Cannabinoid Receptor Type 2 Protein and
mRNA in the Dorsal Root Ganglia of a Rat Neuropathic Pain Model

J 2013

Bilateral Changes of Cannabinoid Receptor Type 2 Protein and mRNA in the Dorsal Root Ganglia of a Rat Neuropathic Pain Model

HRADILOVÁ SVÍŽENSKÁ, Ivana, Václav BRÁZDA, Ilona KLUSÁKOVÁ and Petr DUBOVÝ

Basic information

Original name

Bilateral Changes of Cannabinoid Receptor Type 2 Protein and mRNA in the Dorsal Root Ganglia of a Rat Neuropathic Pain Model

Authors

HRADILOVÁ SVÍŽENSKÁ, Ivana (203 Czech Republic, belonging to the institution), Václav BRÁZDA (203 Czech Republic, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution) and Petr DUBOVÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, LONDON, SAGE PUBLICATIONS LTD, 2013, 0022-1554

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.403

RIV identification code

RIV/00216224:14740/13:00069528

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1369/0022155413491269

UT WoS

000320887100005

Keywords in English

unilateral nerve injury; remote neuroinflammation; satellite glial cells

Abstract

V originále

Cannabinoid receptor type 2 (CB2R) plays a critical role in nociception. In contrast to cannabinoid receptor type 1 ligands, CB2R agonists do not produce undesirable central nervous system effects and thus promise to treat neuropathic pain that is often resistant to medical therapy. In the study presented here, we evaluated the bilateral distribution of the CB2R protein and messenger RNA (mRNA) in rat dorsal root ganglia (DRG) after unilateral peripheral nerve injury using immunohistochemistry, western blot, and in situ hybridization analysis. Unilateral chronic constriction injury (CCI) of the sciatic nerve induced neuropathic pain behavior and bilateral elevation of both CB2R protein and mRNA in lumbar L4-L5 as well as cervical C7-C8 DRG when compared with naive animals. CB2R protein and mRNA were increased not only in DRG neurons but also in satellite glial cells. The fact that changes appear bilaterally and (albeit at a lower level) even in the remote cervical DRG can be related to propagation of neuroinflammation alongside the neuraxis and to the neuroprotective effects of CB2R.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

286154, interní kód MU

Name: SYLICA - Synergies of Life and Material Sciences to Create a New Future (Acronym: SYLICA)

Investor: European Union, Capacities

Citovat

HRADILOVÁ SVÍŽENSKÁ, Ivana, Václav BRÁZDA, Ilona KLUSÁKOVÁ and Petr DUBOVÝ. Bilateral Changes of Cannabinoid Receptor Type 2 Protein and mRNA in the Dorsal Root Ganglia of a Rat Neuropathic Pain Model. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. LONDON: SAGE PUBLICATIONS LTD, 2013, vol. 61, No 7, p. 529-547. ISSN 0022-1554. Available from: https://dx.doi.org/10.1369/0022155413491269.

@article{1124208,
   author = {Hradilová Svíženská, Ivana and Brázda, Václav and Klusáková, Ilona and Dubový, Petr},
   article_location = {LONDON},
   article_number = {7},
   doi = {http://dx.doi.org/10.1369/0022155413491269},
   keywords = {unilateral nerve injury; remote neuroinflammation; satellite glial cells},
   language = {eng},
   issn = {0022-1554},
   journal = {JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY},
   title = {Bilateral Changes of Cannabinoid Receptor Type 2 Protein and mRNA in the Dorsal Root Ganglia of a Rat Neuropathic Pain Model},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/23657829},
   volume = {61},
   year = {2013}
}

TY  - JOUR
ID  - 1124208
AU  - Hradilová Svíženská, Ivana - Brázda, Václav - Klusáková, Ilona - Dubový, Petr
PY  - 2013
TI  - Bilateral Changes of Cannabinoid Receptor Type 2 Protein and mRNA in the Dorsal Root Ganglia of a Rat Neuropathic Pain Model
JF  - JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
VL  - 61
IS  - 7
SP  - 529-547
EP  - 529-547
PB  - SAGE PUBLICATIONS LTD
SN  - 00221554
KW  - unilateral nerve injury
KW  - remote neuroinflammation
KW  - satellite glial cells
UR  - http://www.ncbi.nlm.nih.gov/pubmed/23657829
L2  - http://www.ncbi.nlm.nih.gov/pubmed/23657829
N2  - Cannabinoid receptor type 2 (CB2R) plays a critical role in nociception. In contrast to cannabinoid receptor type 1 ligands, CB2R agonists do not produce undesirable central nervous system effects and thus promise to treat neuropathic pain that is often resistant to medical therapy. In the study presented here, we evaluated the bilateral distribution of the CB2R protein and messenger RNA (mRNA) in rat dorsal root ganglia (DRG) after unilateral peripheral nerve injury using immunohistochemistry, western blot, and in situ hybridization analysis. Unilateral chronic constriction injury (CCI) of the sciatic nerve induced neuropathic pain behavior and bilateral elevation of both CB2R protein and mRNA in lumbar L4-L5 as well as cervical C7-C8 DRG when compared with naive animals. CB2R protein and mRNA were increased not only in DRG neurons but also in satellite glial cells. The fact that changes appear bilaterally and (albeit at a lower level) even in the remote cervical DRG can be related to propagation of neuroinflammation alongside the neuraxis and to the neuroprotective effects of CB2R.
ER  -

HRADILOVÁ SVÍŽENSKÁ, Ivana, Václav BRÁZDA, Ilona KLUSÁKOVÁ and Petr DUBOVÝ. Bilateral Changes of Cannabinoid Receptor Type 2 Protein and mRNA in the Dorsal Root Ganglia of a Rat Neuropathic Pain Model. \textit{JOURNAL OF HISTOCHEMISTRY \&{} CYTOCHEMISTRY}. LONDON: SAGE PUBLICATIONS LTD, 2013, vol.~61, No~7, p.~529-547. ISSN~0022-1554. Available from: https://dx.doi.org/10.1369/0022155413491269.

Detailed Information on Publication Record