BRÁZDA, Václav, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ a Petr DUBOVÝ. Dynamic response to peripheral nerve injury detected by in situ hybridization of IL-6 and its receptor mRNAs in the dorsal root ganglia is not strictly correlated with signs of neuropathic pain. Molecular Pain. London: BIOMED CENTRAL LTD, 2013, roč. 9, č. 42, s. "nestránkováno", 12 s. ISSN 1744-8069. doi:10.1186/1744-8069-9-42.
Další formáty:   BibTeX LaTeX RIS
Základní údaje
Originální název Dynamic response to peripheral nerve injury detected by in situ hybridization of IL-6 and its receptor mRNAs in the dorsal root ganglia is not strictly correlated with signs of neuropathic pain
Autoři BRÁZDA, Václav (203 Česká republika, garant, domácí), Ilona KLUSÁKOVÁ (203 Česká republika, domácí), Ivana HRADILOVÁ SVÍŽENSKÁ (203 Česká republika, domácí) a Petr DUBOVÝ (203 Česká republika, domácí).
Vydání Molecular Pain, London, BIOMED CENTRAL LTD, 2013, 1744-8069.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 30000 3. Medical and Health Sciences
Stát vydavatele Velká Británie
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 3.531
Kód RIV RIV/00216224:14740/13:00069529
Organizační jednotka Středoevropský technologický institut
Doi http://dx.doi.org/10.1186/1744-8069-9-42
UT WoS 000324062000001
Klíčová slova anglicky Cytokines; Contralateral reaction; IL-6 signaling; Neuroinflammation; Neuropathic pain; Unilateral nerve injury
Štítky ok, rivok
Příznaky Recenzováno
Změnil Změnila: Olga Křížová, učo 56639. Změněno: 17. 10. 2013 12:41.
Anotace
Background IL-6 is a typical injury-induced mediator. Together with its receptors, IL-6 contributes to both induction and maintenance of neuropathic pain deriving from changes in activity of primary sensory neurons in dorsal root ganglia (DRG). We used in situ hybridization to provide evidence of IL-6 and IL-6 receptors (IL-6R and gp130) synthesis in DRG along the neuraxis after unilateral chronic constriction injury (CCI) of the sciatic nerve as an experimental model of neuropathic pain. Results All rats operated upon to create unilateral CCI displayed mechanical allodynia and thermal hyperalgesia in ipsilateral hind paws. Contralateral hind paws and forepaws of both sides exhibited only temporal and nonsignificant changes of sensitivity. Very low levels of IL-6 and IL-6R mRNAs were detected in native DRG. IL-6 mRNA was bilaterally increased not only in DRG neurons but also in satellite glial cells (SGC) activated by unilateral CCI. In addition to IL-6 mRNA, substantial increase of IL-6R mRNA expression occurred in DRG neurons and SGC following CCI, while the level of gp130 mRNA remained similar to that of DRG from native rats. Conclusions Here we evidence for the first time increased synthesis of IL-6 and IL-6R in remote cervical DRG nonassociated with the nerve injury. Our results suggest that unilateral CCI of the sciatic nerve induced not only bilateral elevation of IL-6 and IL-6R mRNAs in L4–L5 DRG but also their propagation along the neuraxis to remote cervical DRG as a general neuroinflammatory reaction of the nervous system to local nerve injury without correlation with signs of neuropathic pain. Possible functional involvement of IL-6 signaling is discussed.
Návaznosti
ED1.1.00/02.0068, projekt VaVNázev: CEITEC - central european institute of technology
VytisknoutZobrazeno: 28. 6. 2022 10:57