BRÁZDA, Václav, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ and Petr DUBOVÝ. Dynamic response to peripheral nerve injury detected by in situ hybridization of IL-6 and its receptor mRNAs in the dorsal root ganglia is not strictly correlated with signs of neuropathic pain. Molecular Pain. London: BIOMED CENTRAL LTD, 2013, vol. 9, No 42, p. "nestránkováno", 12 pp. ISSN 1744-8069. doi:10.1186/1744-8069-9-42.
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Basic information
Original name Dynamic response to peripheral nerve injury detected by in situ hybridization of IL-6 and its receptor mRNAs in the dorsal root ganglia is not strictly correlated with signs of neuropathic pain
Authors BRÁZDA, Václav (203 Czech Republic, guarantor, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution), Ivana HRADILOVÁ SVÍŽENSKÁ (203 Czech Republic, belonging to the institution) and Petr DUBOVÝ (203 Czech Republic, belonging to the institution).
Edition Molecular Pain, London, BIOMED CENTRAL LTD, 2013, 1744-8069.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30000 3. Medical and Health Sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.531
RIV identification code RIV/00216224:14740/13:00069529
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1186/1744-8069-9-42
UT WoS 000324062000001
Keywords in English Cytokines; Contralateral reaction; IL-6 signaling; Neuroinflammation; Neuropathic pain; Unilateral nerve injury
Tags ok, rivok
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Changed by Changed by: Olga Křížová, učo 56639. Changed: 17. 10. 2013 12:41.
Abstract
Background IL-6 is a typical injury-induced mediator. Together with its receptors, IL-6 contributes to both induction and maintenance of neuropathic pain deriving from changes in activity of primary sensory neurons in dorsal root ganglia (DRG). We used in situ hybridization to provide evidence of IL-6 and IL-6 receptors (IL-6R and gp130) synthesis in DRG along the neuraxis after unilateral chronic constriction injury (CCI) of the sciatic nerve as an experimental model of neuropathic pain. Results All rats operated upon to create unilateral CCI displayed mechanical allodynia and thermal hyperalgesia in ipsilateral hind paws. Contralateral hind paws and forepaws of both sides exhibited only temporal and nonsignificant changes of sensitivity. Very low levels of IL-6 and IL-6R mRNAs were detected in native DRG. IL-6 mRNA was bilaterally increased not only in DRG neurons but also in satellite glial cells (SGC) activated by unilateral CCI. In addition to IL-6 mRNA, substantial increase of IL-6R mRNA expression occurred in DRG neurons and SGC following CCI, while the level of gp130 mRNA remained similar to that of DRG from native rats. Conclusions Here we evidence for the first time increased synthesis of IL-6 and IL-6R in remote cervical DRG nonassociated with the nerve injury. Our results suggest that unilateral CCI of the sciatic nerve induced not only bilateral elevation of IL-6 and IL-6R mRNAs in L4–L5 DRG but also their propagation along the neuraxis to remote cervical DRG as a general neuroinflammatory reaction of the nervous system to local nerve injury without correlation with signs of neuropathic pain. Possible functional involvement of IL-6 signaling is discussed.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
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