Single-Base Resolution Sequence-Directed Nucleosome Mapping

TRIFONOV, Eduard Nikolajevič and Jan HAPALA. Single-Base Resolution Sequence-Directed Nucleosome Mapping. Israel Journal of Chemistry. WEINHEIM: WILEY-V C H VERLAG GMBH, 2013, vol. 53, 3-4, p. 144-146. ISSN 0021-2148. Available from: https://dx.doi.org/10.1002/ijch.201200074.

Basic information

• Original name: Single-Base Resolution Sequence-Directed Nucleosome Mapping
• Authors: TRIFONOV, Eduard Nikolajevič (376 Israel, guarantor, belonging to the institution) and Jan HAPALA (203 Czech Republic, belonging to the institution).
• Edition: Israel Journal of Chemistry, WEINHEIM, WILEY-V C H VERLAG GMBH, 2013, 0021-2148.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.561
• RIV identification code: RIV/00216224:14740/13:00069571
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1002/ijch.201200074
• UT WoS: 000317859800003
• Keywords in English: DNA; ucleobases; nucleosome positioning; regulation of transcription; TATA box
• Tags: ok, rivok
• Tags: International impact, Reviewed

Abstract

Frequently used nucleosome mapping techniques, both experimental and computational ones, pursue a rather simple task to determine whether a given sequence segment is likely to belong to a nucleosome, thus measuring the nucleosome occupancy along the sequence. A more ambitious task is to determine the position with high resolution, so that not only the approximate translational position of the nucleosome on DNA would be known, but also the rotational setting of the DNA. The rotational setting is important to know since the binding of various transcription factors to the nucleosome DNA crucially depends on the accessibility of the respective recognition sequences. The only experimental technique that provides the highest possible accuracy of the positioning is crystallization of the nucleosomes reconstituted on specific sequences, with subsequent solving of their structures from x-ray diffraction data. Two computational approaches to the accurate positioning of nucleosomes, based on energy calculations and on sequence patterndirected mapping, are currently in different stages of progress.

Links

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations