| NOVÁKOVÁ, Dana, Pavel ŠVEC, Martina KUKLETOVÁ, Lenka ŽÁČKOVÁ and Ivo SEDLÁČEK. Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases. Folia Microbiologica. AV ČR, Institute of Microbiology, 2013, vol. 58, No 6, p. 649-656. ISSN 0015-5632. Available from: https://dx.doi.org/10.1007/s12223-013-0254-6. |
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@article{1124503,
author = {Nováková, Dana and Švec, Pavel and Kukletová, Martina and Žáčková, Lenka and Sedláček, Ivo},
article_number = {6},
doi = {http://dx.doi.org/10.1007/s12223-013-0254-6},
keywords = {biotyping; ribotyping; rep-PCR; caries},
language = {eng},
issn = {0015-5632},
journal = {Folia Microbiologica},
title = {Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases},
volume = {58},
year = {2013}
}
TY - JOUR
ID - 1124503
AU - Nováková, Dana - Švec, Pavel - Kukletová, Martina - Žáčková, Lenka - Sedláček, Ivo
PY - 2013
TI - Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases
JF - Folia Microbiologica
VL - 58
IS - 6
SP - 649-656
EP - 649-656
PB - AV ČR, Institute of Microbiology
SN - 00155632
KW - biotyping
KW - ribotyping
KW - rep-PCR
KW - caries
N2 - Early childhood caries (ECC) has become a serious medical problem worldwide in the last decade. Bacterial microflora of the dental plaque and oral cavity is considered an important factor in the formation and progression of dental caries. The aim of this study was strain typing and comparison of bacterial isolates retrieved from caries lesions and root canal contents of the same teeth. In total, 18 pairs of presumptive streptococci and lactobacilli retrieved from dental caries and root canals isolated from ECC-affected children, were selected on the basis of biotyping results and rep-PCR fingerprinting with (GTG)5 primer. Strain typing was further done using the RiboPrinter microbial characterization system (DuPont Qualicon). The automated ribotyping determined 14 pairs of the strains (77.8 %) to be identical. The results obtained confirmed that identical bacterial strains colonized both the decayed dental surface and the necrotic content of the dental pulp cavity during the cariogenesis. Our finding supports the assumption that bacteria could penetrate through the damaged dental surface to the inner parts of the teeth.
ER -
NOVÁKOVÁ, Dana, Pavel ŠVEC, Martina KUKLETOVÁ, Lenka ŽÁČKOVÁ and Ivo SEDLÁČEK. Evaluation of the strain identity between isolates from caries lesions and root canals in early childhood caries cases. \textit{Folia Microbiologica}. AV ČR, Institute of Microbiology, 2013, vol.~58, No~6, p.~649-656. ISSN~0015-5632. Available from: https://dx.doi.org/10.1007/s12223-013-0254-6.
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