SEDLÁČEK, Ivo, Ondrej ŠEDO, Tereza GELBÍČOVÁ, Andrea VÁVROVÁ PASCUTTI, Eva KROUPOVÁ, Michaela SEDLÁČKOVÁ and Pavel ŠVEC. Characterization of Aeromonas popoffii isolates. In ICCC13. 2013.
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Basic information
Original name Characterization of Aeromonas popoffii isolates
Authors SEDLÁČEK, Ivo (203 Czech Republic, guarantor, belonging to the institution), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Tereza GELBÍČOVÁ (203 Czech Republic, belonging to the institution), Andrea VÁVROVÁ PASCUTTI (203 Czech Republic, belonging to the institution), Eva KROUPOVÁ (203 Czech Republic, belonging to the institution), Michaela SEDLÁČKOVÁ (203 Czech Republic, belonging to the institution) and Pavel ŠVEC (203 Czech Republic, belonging to the institution).
Edition ICCC13, 2013.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/13:00069599
Organization unit Faculty of Science
Keywords in English Aeromonas popoffii; MALDI-TOF MS; ribotyping; phenotyping
Changed by Changed by: Ing. Zdeňka Rašková, učo 140529. Changed: 29/4/2014 09:46.
Abstract
Forty eight A. popoffii-like isolates from water sources (e.g. river freshwater, water pond, underground water and stream water) sampled in Czech Republic and Slovakia during 2008-2012, were characterized by PvuII ribotyping (using RiboPrinter identification system), extensive biochemical testing (tube and plate tests, ENTEROtest 24, Biolog system) and MALDI-TOF MS analysis along with reference strains of A. hydrophila group. These strains were tentatively identified as A. popoffii on the base of the negative sucrose acidification, which is a typical trait of this species. MALDI-TOF MS showed the highest discriminatory power for presumptive A. popoffii isolates and this method seems to be valuable for species identification of A. hydrophila group. RiboPrinter demonstrated great heterogeneity of ribotyping patterns among A. popoffii strains but their identification is impracticable due to the absence of A. popoffii reference patterns in the RiboPrinter identification database. Four presumptive A. popoffii isolates (P3282, P4293, P4571 and P4573) displayed very distinct riboprint profiles. Their further investigation using 16S rRNA and cpn60 genes sequencing assigned these strains as Aeromonas veronii and Aeromonas sobria species.
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EE2.3.20.0183, research and development projectName: Centrum experimentální biomedicíny
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