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@article{1124595,
author = {Šebesta, Marek and Burkovics, Peter and Juhasz, Szilvia and Zhang, Sufang and Szabo, Judit and Lee, Marietta Y. W. T. and Haracska, Lajos and Krejčí, Lumír},
article_location = {AMSTERDAM},
article_number = {9},
doi = {http://dx.doi.org/10.1016/j.dnarep.2013.05.001},
keywords = {TLS polymerases; Homologous recombination; DNA repair synthesis; D-loop; Reconstitution},
language = {eng},
issn = {1568-7864},
journal = {DNA Repair},
title = {Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans},
volume = {12},
year = {2013}
}
TY - JOUR
ID - 1124595
AU - Šebesta, Marek - Burkovics, Peter - Juhasz, Szilvia - Zhang, Sufang - Szabo, Judit - Lee, Marietta Y. W. T. - Haracska, Lajos - Krejčí, Lumír
PY - 2013
TI - Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans
JF - DNA Repair
VL - 12
IS - 9
SP - 691-698
EP - 691-698
PB - ELSEVIER SCIENCE BV
SN - 15687864
KW - TLS polymerases
KW - Homologous recombination
KW - DNA repair synthesis
KW - D-loop
KW - Reconstitution
N2 - Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol delta, TLS polymerases, including Pol eta and Pol kappa, also can extend D-loops. In vivo characterization reveals that Pol eta and Pol kappa are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes.
ER -
ŠEBESTA, Marek, Peter BURKOVICS, Szilvia JUHASZ, Sufang ZHANG, Judit SZABO, Marietta Y. W. T. LEE, Lajos HARACSKA and Lumír KREJČÍ. Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans. \textit{DNA Repair}. AMSTERDAM: ELSEVIER SCIENCE BV, 2013, vol.~12, No~9, p.~691-698. ISSN~1568-7864. Available from: https://dx.doi.org/10.1016/j.dnarep.2013.05.001.
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