Strong Accumulation of Chloroplast DNA in the Y Chromosomes of
Rumex acetosa and Silene latifolia

J 2014

Strong Accumulation of Chloroplast DNA in the Y Chromosomes of Rumex acetosa and Silene latifolia

ŠTEFLOVÁ, Pavlína, Roman HOBZA, Boris VYSKOT and Eduard KEJNOVSKÝ

Basic information

Original name

Strong Accumulation of Chloroplast DNA in the Y Chromosomes of Rumex acetosa and Silene latifolia

Authors

ŠTEFLOVÁ, Pavlína (203 Czech Republic, belonging to the institution), Roman HOBZA (203 Czech Republic), Boris VYSKOT (203 Czech Republic) and Eduard KEJNOVSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Cytogenetic and Genome Research, Basel, Karger, 2014, 1424-8581

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.561

RIV identification code

RIV/00216224:14740/14:00074771

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1159/000355212

UT WoS

000329056600009

Keywords in English

Chloroplast DNA; Rumex acetosa; Sex chromosomes; Silene latifolia

Abstract

V originále

Chloroplast DNA (cpDNA) sequences are often found in plant nuclear genomes, but patterns of their chromosomal distribution are not fully understood. The distribution of cpDNA on the sex chromosomes can only be studied in dioecious plant species possessing heteromorphic sex chromosomes. We reconstructed the whole chloroplast genome of Rumex acetosa (sorrel, XY1Y2 system) from next generation sequencing data. We systematically mapped the chromosomal localization of various regions of cpDNA in R. acetosa and in Silene latifolia (white campion, XY system) using fluorescence in situ hybridization. We found that cpDNA was accumulated on the Y chromosomes of both studied species. In R. acetosa, the entire Y chromosome gathered all parts of cpDNA equally. On the contrary, in S. latifolia, the majority of the cpDNA, corresponding to the single copy regions, was localized in the centromere of the Y chromosome, while the inverted repeat region was present also in other loci. We found a stronger accumulation of cpDNA on the more degenerated Y1 and Y2 chromosomes of R. acetosa than in evolutionary younger S. latifolia Y chromosome. Our data stressed the prominent role of the Y chromosome centromere in cpDNA accumulation.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.20.0045, research and development project

Name: Podpora profesního růstu a mezinárodní integrace výzkumných týmů v oblasti molekulární medicíny

Citovat

ŠTEFLOVÁ, Pavlína, Roman HOBZA, Boris VYSKOT and Eduard KEJNOVSKÝ. Strong Accumulation of Chloroplast DNA in the Y Chromosomes of Rumex acetosa and Silene latifolia. Cytogenetic and Genome Research. Basel: Karger, 2014, vol. 142, No 1, p. 59-65. ISSN 1424-8581. Available from: https://dx.doi.org/10.1159/000355212.

@article{1125390,
   author = {Šteflová, Pavlína and Hobza, Roman and Vyskot, Boris and Kejnovský, Eduard},
   article_location = {Basel},
   article_number = {1},
   doi = {http://dx.doi.org/10.1159/000355212},
   keywords = {Chloroplast DNA; Rumex acetosa; Sex chromosomes; Silene latifolia},
   language = {eng},
   issn = {1424-8581},
   journal = {Cytogenetic and Genome Research},
   title = {Strong Accumulation of Chloroplast DNA in the Y Chromosomes of Rumex acetosa and Silene latifolia},
   url = {http://www.karger.com/Article/FullText/355212},
   volume = {142},
   year = {2014}
}

TY  - JOUR
ID  - 1125390
AU  - Šteflová, Pavlína - Hobza, Roman - Vyskot, Boris - Kejnovský, Eduard
PY  - 2014
TI  - Strong Accumulation of Chloroplast DNA in the Y Chromosomes of Rumex acetosa and Silene latifolia
JF  - Cytogenetic and Genome Research
VL  - 142
IS  - 1
SP  - 59-65
EP  - 59-65
PB  - Karger
SN  - 14248581
KW  - Chloroplast DNA
KW  - Rumex acetosa
KW  - Sex chromosomes
KW  - Silene latifolia
UR  - http://www.karger.com/Article/FullText/355212
L2  - http://www.karger.com/Article/FullText/355212
N2  - Chloroplast DNA (cpDNA) sequences are often found in plant nuclear genomes, but patterns of their chromosomal distribution are not fully understood. The distribution of cpDNA on the sex chromosomes can only be studied in dioecious plant species possessing heteromorphic sex chromosomes. We reconstructed the whole chloroplast genome of Rumex acetosa (sorrel, XY1Y2 system) from next generation sequencing data. We systematically mapped the chromosomal localization of various regions of cpDNA in R. acetosa and in Silene latifolia (white campion, XY system) using fluorescence in situ hybridization. We found that cpDNA was accumulated on the Y chromosomes of both studied species. In R. acetosa, the entire Y chromosome gathered all parts of cpDNA equally. On the contrary, in S. latifolia, the majority of the cpDNA, corresponding to the single copy regions, was localized in the centromere of the Y chromosome, while the inverted repeat region was present also in other loci. We found a stronger accumulation of cpDNA on the more degenerated Y1 and Y2 chromosomes of R. acetosa than in evolutionary younger S. latifolia Y chromosome. Our data stressed the prominent role of the Y chromosome centromere in cpDNA accumulation.
ER  -

ŠTEFLOVÁ, Pavlína, Roman HOBZA, Boris VYSKOT and Eduard KEJNOVSKÝ. Strong Accumulation of Chloroplast DNA in the Y Chromosomes of Rumex acetosa and Silene latifolia. \textit{Cytogenetic and Genome Research}. Basel: Karger, 2014, vol.~142, No~1, p.~59-65. ISSN~1424-8581. Available from: https://dx.doi.org/10.1159/000355212.

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