AREFIN, Badrul, Lucie KUČEROVÁ, Pavel DOBEŠ, Robert MARKUS, Hynek STRNAD, Zhi WANG, Pavel HYRŠL, Michal ŽUROVEC and Ulrich THEOPOLD. Functional studies on transcriptone profiles of Drosophila larvae infected by entomopathogenic nematodes. In Integrated Insect Immunology: From Basic Biology To Environmental Applications. 2013.
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Basic information
Original name Functional studies on transcriptone profiles of Drosophila larvae infected by entomopathogenic nematodes
Name in Czech Functional studies on transcriptone profiles of Drosophila larvae infected by entomopathogenic nematodes
Authors AREFIN, Badrul (752 Sweden), Lucie KUČEROVÁ (203 Czech Republic), Pavel DOBEŠ (203 Czech Republic, belonging to the institution), Robert MARKUS (348 Hungary), Hynek STRNAD (203 Czech Republic), Zhi WANG (752 Sweden), Pavel HYRŠL (203 Czech Republic, guarantor, belonging to the institution), Michal ŽUROVEC (203 Czech Republic) and Ulrich THEOPOLD (752 Sweden).
Edition Integrated Insect Immunology: From Basic Biology To Environmental Applications, 2013.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 30102 Immunology
Country of publisher Poland
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/13:00069819
Organization unit Faculty of Science
Keywords (in Czech) Drosophila; entomopatogenní hlístovky
Keywords in English Drosophila; entomopathogenic nematodes
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavel Dobeš, Ph.D., učo 150960. Changed: 30/1/2016 23:24.
Abstract
Entomopathogenic nematodes (EPN) Heterorhabditis bacteriophora are parasitic worms, which are associated with their gut symbiotic bacteria Photorhabdus luminescens. They are generally lethal to insects and used as biocontrol agents. Nematodes can enter to the host via the mouth, anus, spiracles or penetrating through the cuticle. Once they entered into host, they release their gut bacteria and then work in concert with the bacteria to kill the host. Nematode infection in Drosophila is a natural infection, in contrast to many kinds of bacterial infection (through injection). Drosophila lacks an adaptive immune system of the vertebrate style and therefore relies on innate immunity to combat infection against different invaders like bacteria, fungi, viruses and other parasites. Until now, our knowledge on Drosophila immunity mostly comes from studies of bacterial and fungal infections. To obtain a complete picture of Drosophila response to EPN, we performed a genome-wide transcriptional analysis of the Drosophila larvae (approximately 88 hour after egg laying) infected by EPN. We compared the transcriptome profile between infected and non-infected larvae. A total of 642 transcripts were found significantly differentially regulated upon nematode infection. Gene ontology (GO) analysis of the top 100 most significantly upregulated genes identified one-quarter immune-related genes. To test their contribution to the response against nematodes, we used RNAi lines and mutants for candidate genes identified in the arrays and examined the viability of such larvae upon EPN infection. As a criterion for selecting candidate defense genes from the list of induced transcripts we focused on those for which a function in immunity had been established in other infection models, as well as on their paralogs, which had not yet been implicated in immune reactions.
Abstract (in Czech)
Entomopathogenic nematodes (EPN) Heterorhabditis bacteriophora are parasitic worms, which are associated with their gut symbiotic bacteria Photorhabdus luminescens. They are generally lethal to insects and used as biocontrol agents. Nematodes can enter to the host via the mouth, anus, spiracles or penetrating through the cuticle. Once they entered into host, they release their gut bacteria and then work in concert with the bacteria to kill the host. Nematode infection in Drosophila is a natural infection, in contrast to many kinds of bacterial infection (through injection). Drosophila lacks an adaptive immune system of the vertebrate style and therefore relies on innate immunity to combat infection against different invaders like bacteria, fungi, viruses and other parasites. Until now, our knowledge on Drosophila immunity mostly comes from studies of bacterial and fungal infections. To obtain a complete picture of Drosophila response to EPN, we performed a genome-wide transcriptional analysis of the Drosophila larvae (approximately 88 hour after egg laying) infected by EPN. We compared the transcriptome profile between infected and non-infected larvae. A total of 642 transcripts were found significantly differentially regulated upon nematode infection. Gene ontology (GO) analysis of the top 100 most significantly upregulated genes identified one-quarter immune-related genes. To test their contribution to the response against nematodes, we used RNAi lines and mutants for candidate genes identified in the arrays and examined the viability of such larvae upon EPN infection. As a criterion for selecting candidate defense genes from the list of induced transcripts we focused on those for which a function in immunity had been established in other infection models, as well as on their paralogs, which had not yet been implicated in immune reactions.
Links
CZ.1.07/2.3.00/30.0009, interní kód MU
(CEP code: EE2.3.30.0009)		Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci (Acronym: Postdoc I.)
Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development
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