USTIANENKO, Dmytro, Dominika HROŠŠOVÁ, David POTĚŠIL, Kateřina CHALUPNÍKOVÁ, Kristýna HRAZDILOVÁ, Jiří PACHERNÍK, Kateřina CETKOVSKÁ, Stjepan ULDRIJAN, Zbyněk ZDRÁHAL and Štěpánka VAŇÁČOVÁ. Mammalian DIS3L2 exoribonuclease targets the uridylated precursors of let-7 miRNAs. RNA. New York: Cold Spring Harbor Laboratory Press, 2013, vol. 19, No 12, p. 1632-1638. ISSN 1355-8382. doi:10.1261/rna.040055.113.
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Basic information
Original name Mammalian DIS3L2 exoribonuclease targets the uridylated precursors of let-7 miRNAs
Authors USTIANENKO, Dmytro (804 Ukraine, belonging to the institution), Dominika HROŠŠOVÁ (703 Slovakia, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution), Kateřina CHALUPNÍKOVÁ (203 Czech Republic, belonging to the institution), Kristýna HRAZDILOVÁ (203 Czech Republic, belonging to the institution), Jiří PACHERNÍK (203 Czech Republic, belonging to the institution), Kateřina CETKOVSKÁ (203 Czech Republic, belonging to the institution), Stjepan ULDRIJAN (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution) and Štěpánka VAŇÁČOVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition RNA, New York, Cold Spring Harbor Laboratory Press, 2013, 1355-8382.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 4.622
RIV identification code RIV/00216224:14740/13:00066446
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1261/rna.040055.113
UT WoS 000327445700004
Keywords in English DIS3L2; RNA degradation; RNA uridylation; let-7 miRNA
Tags ok, podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 17. 1. 2014 15:38.
Abstract
The mechanisms of gene expression regulation by miRNAs have been extensively studied. However, the regulation of miRNA function and decay has long remained enigmatic. Only recently, 3'uridylation via LIN28A-TUT4/7 has been recognized as an essential component controlling the biogenesis of let-7 miRNAs in stem cells. Although uridylation has been generally implicated in miRNA degradation, the nuclease responsible has remained unknown. Here, we identify the Perlman syndromeassociated protein DIS3L2 as an oligo(U)-binding and processing exoribonuclease that specifically targets uridylated pre-let-7 in vivo. This study establishes DIS3L2 as the missing component of the LIN28-TUT4/7-DIS3L2 pathway required for the repression of let-7 in pluripotent cells.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
GAP305/11/1095, research and development projectName: Funkční a biochemická charakterizace proteinu Dis3L2, třetího lidského homologu hlavní kvasinkové exosomové nukleázy Dis3p
Investor: Czech Science Foundation, Functional and biochemical characterization of Dis3L2, the third mammalian homolog of the key yeast exosome nuclease Dis3p
GBP206/12/G151, research and development projectName: Centrum nových přístupů k bioanalýze a molekulární diagnostice
GBP305/12/G034, research and development projectName: Centrum biologie RNA
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