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@article{1126888,
author = {Lermontova, Inna and Kuhlmann, Markus and Friedel, Swetlana and Rutten, Twan and Heckmann, Stefan and Sandmann, Michael and Demidov, Dmitri and Schubert, Veit and Schubert, Ingo},
article_number = {9},
doi = {http://dx.doi.org/10.1105/tpc.113.114736},
keywords = {KINETOCHORE NULL2; Arabidopsis thaliana; centromere},
language = {eng},
issn = {1040-4651},
journal = {Plant Cell},
title = {Arabidopsis KINETOCHORE NULL2 Is an Upstream Component for Centromeric Histone H3 Variant cenH3 Deposition at Centromeres},
url = {http://www.plantcell.org/content/early/2013/09/05/tpc.113.114736.abstract},
volume = {25},
year = {2013}
}
TY - JOUR
ID - 1126888
AU - Lermontova, Inna - Kuhlmann, Markus - Friedel, Swetlana - Rutten, Twan - Heckmann, Stefan - Sandmann, Michael - Demidov, Dmitri - Schubert, Veit - Schubert, Ingo
PY - 2013
TI - Arabidopsis KINETOCHORE NULL2 Is an Upstream Component for Centromeric Histone H3 Variant cenH3 Deposition at Centromeres
JF - Plant Cell
VL - 25
IS - 9
SP - 3389-3404
EP - 3389-3404
PB - AMER SOC PLANT BIOLOGISTS
SN - 10404651
KW - KINETOCHORE NULL2
KW - Arabidopsis thaliana
KW - centromere
UR - http://www.plantcell.org/content/early/2013/09/05/tpc.113.114736.abstract
L2 - http://www.plantcell.org/content/early/2013/09/05/tpc.113.114736.abstract
N2 - The centromeric histone H3 variant cenH3 is an essential centromeric protein required for assembly, maintenance, and proper function of kinetochores during mitosis and meiosis. We identified a KINETOCHORE NULL2 (KNL2) homolog in Arabidopsis thaliana and uncovered features of its role in cenH3 loading at centromeres. We show that Arabidopsis KNL2 colocalizes with cenH3 and is associated with centromeres during all stages of the mitotic cell cycle, except from metaphase to mid-anaphase. KNL2 is regulated by the proteasome degradation pathway. The KNL2 promoter is mainly active in meristematic tissues, similar to the cenH3 promoter. A knockout mutant for KNL2 shows a reduced level of cenH3 expression and reduced amount of cenH3 protein at chromocenters of meristematic nuclei, anaphase bridges during mitosis, micronuclei in pollen tetrads, and 30%seed abortion. Moreover, knl2 mutant plants display reduced expression of suppressor of variegation 3-9 homologs2, 4, and 9 and reduced DNA methylation, suggesting an impact of KNL2 on the epigenetic environment for centromere maintenance.
ER -
LERMONTOVA, Inna, Markus KUHLMANN, Swetlana FRIEDEL, Twan RUTTEN, Stefan HECKMANN, Michael SANDMANN, Dmitri DEMIDOV, Veit SCHUBERT and Ingo SCHUBERT. Arabidopsis KINETOCHORE NULL2 Is an Upstream Component for Centromeric Histone H3 Variant cenH3 Deposition at Centromeres. \textit{Plant Cell}. AMER SOC PLANT BIOLOGISTS, 2013, vol.~25, No~9, p.~3389-3404. ISSN~1040-4651. Available from: https://dx.doi.org/10.1105/tpc.113.114736.
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