The role of ATP-binding transporters associated with multi-drug
resistance in stem cells

k 2013

The role of ATP-binding transporters associated with multi-drug resistance in stem cells

LÁNOVÁ, Martina, Josef VEČEŘA, Jan KUČERA, Jiřina MEDALOVÁ, Jiří PACHERNÍK et. al.

Basic information

Original name

The role of ATP-binding transporters associated with multi-drug resistance in stem cells

Authors

LÁNOVÁ, Martina (203 Czech Republic, belonging to the institution), Josef VEČEŘA (203 Czech Republic, belonging to the institution), Jan KUČERA (203 Czech Republic, belonging to the institution), Jiřina MEDALOVÁ (203 Czech Republic, belonging to the institution) and Jiří PACHERNÍK (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Conference Analytical Cytometry VII Mikulov 2013, 2013

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

30105 Physiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/13:00070040

Organization unit

Faculty of Science

ISBN

978-80-905449-2-5

Keywords in English

ABC-transporters neural stem cells

Změněno: 11/3/2015 10:52, RNDr. Josef Večeřa, Ph.D.

Abstract

V originále

ATP-binding transporters (ABC-t) play various roles in regulation organism function and homeostasis from prokaryota to mammals. ABC-t mediate transport of mainly lipophilic substances through cellular membranes. Some ABC-t are important in cell protection against endogenous and importantly also exogenous toxins. These transporters are called ABC-t associated with multi-drug resistance (ABC-t/MDR), according to their role in resistance of tumor cells to pharmacotherapy. ABC-t/MDR are also over-expressed in stem cells, where their protective role is expected, too. Particularly, ABCB1, ABCC1, and ABCG2 are common ABC-t/MDR expressed in stem cells. However, substrates of ABC-t/MDR are not only toxins, but also important signaling molecules as well leukotrienes and/or glutathione conjugates and porphyrins, which mediated balance in intracellular oxidation-reduction processing. Thus we hypothesize the role of ABC-t/MDR also in regulation of stem cells fate. To test this hypothesis we analyzed effect of modulation of ABC-t/MDR activity in embryonic and neural stem cells. We observed that ABCC1 and ABCG2 are the most expressed ABC-t/MDR in our tested stem cells. Importantly, inhibition of these ABC-t/MDR leads to decreasing of stemness and induction of differentiation in both embryonic and neural stem cells. Analysis of mechanism of observed effect and identification of studying ABC-t/MDR substrates, which may be responsible for this effect, are in progression.

Links

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

Citovat

LÁNOVÁ, Martina, Josef VEČEŘA, Jan KUČERA, Jiřina MEDALOVÁ and Jiří PACHERNÍK. The role of ATP-binding transporters associated with multi-drug resistance in stem cells. In International Conference Analytical Cytometry VII Mikulov 2013. 2013. ISBN 978-80-905449-2-5.

@proceedings{1128405,
   author = {Lánová, Martina and Večeřa, Josef and Kučera, Jan and Medalová, Jiřina and Pacherník, Jiří},
   booktitle = {International Conference Analytical Cytometry VII Mikulov 2013},
   keywords = {ABC-transporters neural stem cells},
   language = {eng},
   isbn = {978-80-905449-2-5},
   title = {The role of ATP-binding transporters associated with multi-drug resistance in stem cells},
   year = {2013}
}

TY  - CONF
ID  - 1128405
AU  - Lánová, Martina - Večeřa, Josef - Kučera, Jan - Medalová, Jiřina - Pacherník, Jiří
PY  - 2013
TI  - The role of ATP-binding transporters associated with multi-drug resistance in stem cells
SN  - 9788090544925
KW  - ABC-transporters neural stem cells
N2  - ATP-binding transporters (ABC-t) play various roles in regulation organism function and homeostasis from prokaryota to mammals. ABC-t mediate transport of mainly lipophilic substances through cellular membranes. Some ABC-t are important in cell protection against endogenous and importantly also exogenous toxins. These transporters are called ABC-t associated with multi-drug resistance (ABC-t/MDR), according to their role in resistance of tumor cells to pharmacotherapy. ABC-t/MDR are also over-expressed in stem cells, where their protective role is expected, too. Particularly, ABCB1, ABCC1, and ABCG2 are common ABC-t/MDR expressed in stem cells. However, substrates of ABC-t/MDR are not only toxins, but also important signaling molecules as well leukotrienes and/or glutathione conjugates and porphyrins, which mediated balance in intracellular oxidation-reduction processing. Thus we hypothesize the role of ABC-t/MDR also in regulation of stem cells fate. To test this hypothesis we analyzed effect of modulation of ABC-t/MDR activity in embryonic and neural stem cells. We observed that ABCC1 and ABCG2 are the most expressed ABC-t/MDR in our tested stem cells. Importantly, inhibition of these ABC-t/MDR leads to decreasing of stemness and induction of differentiation in both embryonic and neural stem cells. Analysis of mechanism of observed effect and identification of studying ABC-t/MDR substrates, which may be responsible for this effect, are in progression.
ER  -

LÁNOVÁ, Martina, Josef VEČEŘA, Jan KUČERA, Jiřina MEDALOVÁ and Jiří PACHERNÍK. The role of ATP-binding transporters associated with multi-drug resistance in stem cells. In \textit{International Conference Analytical Cytometry VII Mikulov 2013}. 2013. ISBN~978-80-905449-2-5.

Detailed Information on Publication Record