ČERNOHLÁVKOVÁ, Jitka, Ivo SEDLÁČEK and Eva KROUPOVÁ. Taxonomic characterisation of Aeromonas hydrophila complex. In XXXII Annual Meeting of the European Culture Collections' Organization (ECCO). 2013.
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Basic information
Original name Taxonomic characterisation of Aeromonas hydrophila complex
Name in Czech taxonomická charakterizace komplexu Aeromonas hydrophila
Authors ČERNOHLÁVKOVÁ, Jitka (203 Czech Republic, guarantor, belonging to the institution), Ivo SEDLÁČEK (203 Czech Republic) and Eva KROUPOVÁ (203 Czech Republic).
Edition XXXII Annual Meeting of the European Culture Collections' Organization (ECCO), 2013.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/13:00070051
Organization unit Faculty of Science
Keywords in English taxonomy Aeromonas hydrophila biotyping ribotyping SDS-page
Changed by Changed by: Ing. Zdeňka Rašková, učo 140529. Changed: 29/4/2014 10:31.
Abstract
The aim of this study was to clarify taxonomic position of A. salmonicida spp. strains deposited at Czech Collection of Microorganism within the “A. hydrophila” complex. In total 11 strains of A. salmonicida and 6 type strains of A. bestiarum, A. hydrophila subsp. hydrophila, A. hydrophila subsp. dhakensis, A. hydrophila subsp. ranae, A. aquariorum and A. popoffii were characterised using biotyping, whole-cell protein profile analysis, ribotyping and gene sequencing (cnp60). Two unknown isolates of Aeromonas sp. from water were included into the analysis. Results of whole-cell protein analysis distinguished different species of “A. hydrophila” complex into independent clusters. A. salmonicida subsp. masoucida and A. bestiarum were clearly separated from the others strains at the similarity level less than 50%. A. hydrophila subsp. dhakensis and A. aquariorum clustered together with the 86% similarity, which support a theory that these species represent one taxon.Genetic analysis based on ribotyping also separated the strains into independent subclusters of A. bestiarum and A. hydrophila subsp. hydrophila (52% similarity), A. salmonicida subsp. masoucida and A. hydrophila subsp. ranae (64% similarity). A. salmonicida subsp. salmonicida were clustered together with A. salmonicida subsp. achromogenes (84% similarity) and one isolate of Aeromonas sp.Results of ribotyping also showed that A. hydrophila subsp. dhakensis and A. aquariorum are closely related, however the similarity was less than 60%.However further genetic analyses (e.g. gyrB and rpoD gene sequencing) or DNA-DNA hybridization would be necessary to reliable identification and verification of taxonomic position of this complex.
Links
EE2.3.20.0183, research and development projectName: Centrum experimentální biomedicíny
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