An induced pluripotent stem cell model of hypoplastic left
heart syndrome (HLHS) reveals multiple expression and
functional differences in HLHS derived cardiac myocytes

J 2014

An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS derived cardiac myocytes

JIANG, Yan, Saba HABIBOLLAH, Katarzyna TILGNER, Joseph COLLIN, Tomáš BÁRTA et. al.

Basic information

Original name

An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS derived cardiac myocytes

Authors

JIANG, Yan (826 United Kingdom of Great Britain and Northern Ireland), Saba HABIBOLLAH (826 United Kingdom of Great Britain and Northern Ireland), Katarzyna TILGNER (826 United Kingdom of Great Britain and Northern Ireland), Joseph COLLIN (826 United Kingdom of Great Britain and Northern Ireland), Tomáš BÁRTA (203 Czech Republic), Jumana Yousuf AL-AAMA (826 United Kingdom of Great Britain and Northern Ireland), Lenka TESAŘOVÁ (203 Czech Republic, guarantor, belonging to the institution), Rafiqul HUSSAIN (826 United Kingdom of Great Britain and Northern Ireland), Andrew TRAFFORD (826 United Kingdom of Great Britain and Northern Ireland), Graham KIRKWOOD (826 United Kingdom of Great Britain and Northern Ireland), Evelyne SERNAGOR (826 United Kingdom of Great Britain and Northern Ireland), Cyril ELEFTHERIOU (826 United Kingdom of Great Britain and Northern Ireland), Stefan PRZYBORSKI (826 United Kingdom of Great Britain and Northern Ireland), Miodrag STOJKOVIC (826 United Kingdom of Great Britain and Northern Ireland), Majlinda LAKO (826 United Kingdom of Great Britain and Northern Ireland), Bernard KEAVNEY (826 United Kingdom of Great Britain and Northern Ireland) and Lyle ARMSTRONG (826 United Kingdom of Great Britain and Northern Ireland)

Edition

Stem cells translational medicine, 2014, 2157-6564

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.709

RIV identification code

RIV/00216224:14330/14:00074816

Organization unit

Faculty of Informatics

DOI

http://dx.doi.org/10.5966/sctm.2013-0105

UT WoS

000333938200007

Keywords in English

Hypoplastic left heart syndrome; induced pluripotent stem cells; cardiac myocytes; cardiac development; pluripotent stem cell differentiation

Abstract

V originále

Hypoplastic left heart syndrome (HLHS) is a serious congenital cardiovascular malformation resulting in hypoplasia or atresia of the left ventricle, ascending aorta, aortic and mitral valves. Diminished flow through the left side of the heart is clearly a key contributor to the condition, but any myocardial susceptibility component is as yet undefined. Using recent advances in the field of induced pluripotent stem cells (iPSC), we have been able to generate an iPSC model of HLHS malformation and characterise the properties of cardiac myocytes (CM) differentiated from these and control-iPSC lines. Differentiation of HLHS-iPSC to cardiac lineages revealed changes in the expression of key cardiac markers and a lower ability to give rise to beating clusters when compared to control-iPSC and human embryonic stem cells (hESC). HLHS-iPSC derived CM show a lower level of myofibrillar organisation, persistence of an fetal gene expression pattern, changes in commitment to ventricular versus atriallineages and display different calcium transient patterns and electrophysiological responses to caffeine and beta-adrenergic antagonists when compared to hESC- and control-iPSC derived CM, suggesting that alternative mechanisms to release calcium from intracellular stores such as the inositol triphosphate receptor may exist in HLHS in addition to the ryanodine receptor thought to function in control-iPSC derived CM. Together our findings demonstrate that CM derived from an HLHS patient demonstrate a number of marker expression and functional differences to hESC/control iPSC derived CM, thus providing some evidence that cardiomyocyte-specific factors may influence the risk of HLHS.

Links

CZ.1.07/2.3.00/30.0030, interní kód MU

Name: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development

Citovat

JIANG, Yan, Saba HABIBOLLAH, Katarzyna TILGNER, Joseph COLLIN, Tomáš BÁRTA, Jumana Yousuf AL-AAMA, Lenka TESAŘOVÁ, Rafiqul HUSSAIN, Andrew TRAFFORD, Graham KIRKWOOD, Evelyne SERNAGOR, Cyril ELEFTHERIOU, Stefan PRZYBORSKI, Miodrag STOJKOVIC, Majlinda LAKO, Bernard KEAVNEY and Lyle ARMSTRONG. An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS derived cardiac myocytes. Stem cells translational medicine. 2014, vol. 3, No 4, p. 416-423. ISSN 2157-6564. Available from: https://dx.doi.org/10.5966/sctm.2013-0105.

@article{1129955,
   author = {Jiang, Yan and Habibollah, Saba and Tilgner, Katarzyna and Collin, Joseph and Bárta, Tomáš and AlandAama, Jumana Yousuf and Tesařová, Lenka and Hussain, Rafiqul and Trafford, Andrew and Kirkwood, Graham and Sernagor, Evelyne and Eleftheriou, Cyril and Przyborski, Stefan and Stojkovic, Miodrag and Lako, Majlinda and Keavney, Bernard and Armstrong, Lyle},
   article_number = {4},
   doi = {http://dx.doi.org/10.5966/sctm.2013-0105},
   keywords = {Hypoplastic left heart syndrome; induced pluripotent stem cells; cardiac myocytes; cardiac development; pluripotent stem cell differentiation},
   language = {eng},
   issn = {2157-6564},
   journal = {Stem cells translational medicine},
   title = {An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS derived cardiac myocytes},
   volume = {3},
   year = {2014}
}

TY  - JOUR
ID  - 1129955
AU  - Jiang, Yan - Habibollah, Saba - Tilgner, Katarzyna - Collin, Joseph - Bárta, Tomáš - Al-Aama, Jumana Yousuf - Tesařová, Lenka - Hussain, Rafiqul - Trafford, Andrew - Kirkwood, Graham - Sernagor, Evelyne - Eleftheriou, Cyril - Przyborski, Stefan - Stojkovic, Miodrag - Lako, Majlinda - Keavney, Bernard - Armstrong, Lyle
PY  - 2014
TI  - An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS derived cardiac myocytes
JF  - Stem cells translational medicine
VL  - 3
IS  - 4
SP  - 416-423
EP  - 416-423
SN  - 21576564
KW  - Hypoplastic left heart syndrome
KW  - induced pluripotent stem cells
KW  - cardiac myocytes
KW  - cardiac development
KW  - pluripotent stem cell differentiation
N2  - Hypoplastic left heart syndrome (HLHS) is a serious congenital cardiovascular malformation resulting in hypoplasia or atresia of the left ventricle, ascending aorta, aortic and mitral valves. Diminished flow through the left side of the heart is clearly a key contributor to the condition, but any myocardial susceptibility component is as yet undefined. Using recent advances in the field of induced pluripotent stem cells (iPSC), we have been able to generate an iPSC model of HLHS malformation and characterise the properties of cardiac myocytes (CM) differentiated from these and control-iPSC lines. Differentiation of HLHS-iPSC to cardiac lineages revealed changes in the expression of key cardiac markers and a lower ability to give rise to beating clusters when compared to control-iPSC and human embryonic stem cells (hESC). HLHS-iPSC derived CM show a lower level of myofibrillar organisation, persistence of an fetal gene expression pattern, changes in commitment to ventricular versus atriallineages and display different calcium transient patterns and electrophysiological responses to caffeine and beta-adrenergic antagonists when compared to hESC- and control-iPSC derived CM, suggesting that alternative mechanisms to release calcium from intracellular stores such as the inositol triphosphate receptor may exist in HLHS in addition to the ryanodine receptor thought to function in control-iPSC derived CM. Together our findings demonstrate that CM derived from an HLHS patient demonstrate a number of marker expression and functional differences to hESC/control iPSC derived CM, thus providing some evidence that cardiomyocyte-specific factors may influence the risk of HLHS.
ER  -

JIANG, Yan, Saba HABIBOLLAH, Katarzyna TILGNER, Joseph COLLIN, Tomáš BÁRTA, Jumana Yousuf AL-AAMA, Lenka TESAŘOVÁ, Rafiqul HUSSAIN, Andrew TRAFFORD, Graham KIRKWOOD, Evelyne SERNAGOR, Cyril ELEFTHERIOU, Stefan PRZYBORSKI, Miodrag STOJKOVIC, Majlinda LAKO, Bernard KEAVNEY and Lyle ARMSTRONG. An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS derived cardiac myocytes. \textit{Stem cells translational medicine}. 2014, vol.~3, No~4, p.~416-423. ISSN~2157-6564. Available from: https://dx.doi.org/10.5966/sctm.2013-0105.

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