RENČIUK, Daniel, Olivier BLACQUE, Michaela VORLÍČKOVÁ a Bernhard SPINGLER. Crystal structures of B-DNA dodecamer containing the epigenetic modifications 5-hydroxymethylcytosine or 5-methylcytosine. Nucleic Acids Research. Oxford: Oxford University Press, 2013, roč. 41, č. 21, s. 9891-9900. ISSN 0305-1048. doi:10.1093/nar/gkt738.
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Základní údaje
Originální název Crystal structures of B-DNA dodecamer containing the epigenetic modifications 5-hydroxymethylcytosine or 5-methylcytosine
Autoři RENČIUK, Daniel (203 Česká republika), Olivier BLACQUE (756 Švýcarsko), Michaela VORLÍČKOVÁ (203 Česká republika, garant, domácí) a Bernhard SPINGLER (756 Švýcarsko).
Vydání Nucleic Acids Research, Oxford, Oxford University Press, 2013, 0305-1048.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10600 1.6 Biological sciences
Stát vydavatele Velká Británie
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 8.808
Kód RIV RIV/00216224:14740/13:00070285
Organizační jednotka Středoevropský technologický institut
Doi http://dx.doi.org/10.1093/nar/gkt738
UT WoS 000327541800031
Klíčová slova anglicky NUCLEIC-ACID STRUCTURES; CIRCULAR-DICHROISM; DEOXYRIBONUCLEIC-ACID; ATOMIC-RESOLUTION; PROTEIN CRYSTALS; BINDING-SITES; MAMMALIAN DNA; CRYSTALLIZATION; PARAMETERS; SOFTWARE
Štítky ok, rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Olga Křížová, učo 56639. Změněno: 31. 12. 2013 06:11.
Anotace
5-Hydroxymethylcytosine (5-hmC) was recently identified as a relatively frequent base in eukaryotic genomes. Its physiological function is still unclear, but it is supposed to serve as an intermediate in DNA de novo demethylation. Using X-ray diffraction, we solved five structures of four variants of the d(CGCG AATTCGCG) dodecamer, containing either 5-hmC or 5-methylcytosine (5-mC) at position 3 or at position 9. The observed resolutions were between 1.42 and 1.99A. Cytosine modification in all cases influences neither the whole B-DNA double helix structure nor the modified base pair geometry. The additional hydroxyl group of 5-hmC with rotational freedom along the C5-C5A bond is preferentially oriented in the 30 direction. A comparison of thermodynamic properties of the dodecamers shows no effect of 5- mC modification and a sequence-dependent only slight destabilizing effect of 5-hmC modification. Also taking into account the results of a previous functional study /Munzel et al. (2011)(Improved synthesis and mutagenicity of oligonucleotides containing 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxylcytosine. Chem. Eur. J., 17, 13782-13788)/, we conclude that the 5 position of cytosine is an ideal place to encode epigenetic information. Like this, neither the helical structure nor the thermodynamics are changed, and polymerases cannot distinguish 5- hmC and 5-mC from unmodified cytosine, all these effects are making the former ones non-mutagenic.
Návaznosti
ED1.1.00/02.0068, projekt VaVNázev: CEITEC - central european institute of technology
VytisknoutZobrazeno: 8. 8. 2022 16:36