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@article{1131191,
author = {Smetana, Jan and Kryukova, Elena Vladimirovna and Kryukov, Fedor and Němec, Pavel and Grešliková, Henrieta and Kupská, Renata and Mikulášová, Aneta and Ihnatová, Ivana and Hájek, Roman and Kuglík, Petr},
article_location = {Bratislava},
article_number = {1},
doi = {http://dx.doi.org/10.4149/neo_2014_008},
keywords = {multiple myeloma; CD19+; CD138+; cytogenetics; array-CGH; FISH},
language = {eng},
issn = {0028-2685},
journal = {Neoplasma},
title = {Incidence of cytogenetic aberrations in two B lineage subpopulations in multiple myeloma patients analyzed by combination of whole-genome profiling and FISH},
volume = {61},
year = {2014}
}
TY - JOUR
ID - 1131191
AU - Smetana, Jan - Kryukova, Elena Vladimirovna - Kryukov, Fedor - Němec, Pavel - Grešliková, Henrieta - Kupská, Renata - Mikulášová, Aneta - Ihnatová, Ivana - Hájek, Roman - Kuglík, Petr
PY - 2014
TI - Incidence of cytogenetic aberrations in two B lineage subpopulations in multiple myeloma patients analyzed by combination of whole-genome profiling and FISH
JF - Neoplasma
VL - 61
IS - 1
SP - 48-55
EP - 48-55
PB - Slovenská akademie vied
SN - 00282685
KW - multiple myeloma
KW - CD19+
KW - CD138+
KW - cytogenetics
KW - array-CGH
KW - FISH
N2 - Multiple myeloma (MM) is an incurable malignant disease of the terminal developmental stage of B-lymphocytes. While genetic heterogeneity of MM is widely described, little is known about its genetic basis as well as primary damage during plasma cells (PC) development. In this study, we focused on genome-wide screening of DNA copy number changes using oligonucleotide-based array-CGH together with I-FISH of the IgH locus rearrangements in pair samples of bone marrow B-cells (CD19+) and CD138+ PC from newly diagnosed MM patients. The IgH disruption was found in 8.9% (4/45) of CD19+ samples and in 57.8% (26/45) of CD138+ samples. The genomic profiling using array-CGH identified copy number alterations (CNAs) in 10% (2/20) of CD19+ samples in regions known to be important for MM pathogenesis. In contrast, we found CNAs in 100% (16/16) of CD138+ samples. Most common chromosomal abnormalities were trisomies of odd-numbered chromosomes (3, 5, 7, 9, 11, 15, 19 and 21), gain 1q, gain Xq and monosomy of chromosome 13. We did not find any correlation between incidence of CNAs in CD19+ and CD138+ cells. In conclusion, effective utilization of FISH and array-CGH can identify genetic lesions in premalignant stages leading to better understanding and characterization of MM.
ER -
SMETANA, Jan, Elena Vladimirovna KRYUKOVA, Fedor KRYUKOV, Pavel NĚMEC, Henrieta GREŠLIKOVÁ, Renata KUPSKÁ, Aneta MIKULÁŠOVÁ, Ivana IHNATOVÁ, Roman HÁJEK a Petr KUGLÍK. Incidence of cytogenetic aberrations in two B lineage subpopulations in multiple myeloma patients analyzed by combination of whole-genome profiling and FISH. \textit{Neoplasma}. Bratislava: Slovenská akademie vied, 2014, roč.~61, č.~1, s.~48-55. ISSN~0028-2685. Dostupné z: https://dx.doi.org/10.4149/neo\_{}2014\_{}008.
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