MIDLIK, Adam, Kristýna PĚNČÍKOVÁ, Ivana KUŠNYEROVÁ, Gabriela DOVRTĚLOVÁ, Kristýna NOSKOVÁ, Jan JUŘICA, Eva TÁBORSKÁ and Ondřej PEŠ. LIQUID CHROMATOGRAPHY MASS SPECTROMETRY OF SELECTED BENZOPHENANTHRIDINE ALKALOIDS INCUBATED WITH RAT LIVER MICROSOMES. Chemické listy. Česká společnost chemická, 2013, vol. 107, Special Issue, Supplement 3, p. S405-S406, 2 pp. ISSN 0009-2770.
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Basic information
Original name LIQUID CHROMATOGRAPHY MASS SPECTROMETRY OF SELECTED BENZOPHENANTHRIDINE ALKALOIDS INCUBATED WITH RAT LIVER MICROSOMES
Authors MIDLIK, Adam (703 Slovakia, belonging to the institution), Kristýna PĚNČÍKOVÁ (203 Czech Republic, belonging to the institution), Ivana KUŠNYEROVÁ (703 Slovakia, belonging to the institution), Gabriela DOVRTĚLOVÁ (203 Czech Republic, belonging to the institution), Kristýna NOSKOVÁ (203 Czech Republic, belonging to the institution), Jan JUŘICA (203 Czech Republic, belonging to the institution), Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution) and Ondřej PEŠ (203 Czech Republic, guarantor, belonging to the institution).
Edition Chemické listy, Česká společnost chemická, 2013, 0009-2770.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 0.196
RIV identification code RIV/00216224:14110/13:00070341
Organization unit Faculty of Medicine
UT WoS 000328730800049
Keywords in English LC MS; QBA; benzophenanthridine; rat liver microsomes; metabolite
Tags EL OK, HPLC, mass spectrometry, podil, separation
Tags International impact, Reviewed
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 10. 3. 2014 15:15.
Abstract
Quaternary benzo[c]phenantridine alkaloids (QBAs) belong to a subgroup of isoquinoline alkaloids. They include major sanguinarine (SA) and chelerythrine (CHE) as well as minor QBAs chelirubine (CR), sanguilutine (SL), sanguirubine (SR), chelilutine (CL), and macarpine (MA). The effects of QBAs on biological systems have been studied extensively, especially for commercially available SA and CHE. Limited availability of the minor pentasubstituted QBAs and hexasubstituted MA reduces data concerning their biological effects. Although the QBA molecular structures are very similar, the mechanism of their action on the molecular level may be different. As pharmacological studies imply that even a small change in the structure may trigger a significant difference in pharmacological activity, it cannot be simply accepted that metabolic routes of all QBAs will be analogous. The metabolic fate of minor QBAs should be intensively studied in order to identify their metabolites, to reveal their toxicity and/or alternate biological effects in different pathways. An approach to reveal the metabolic fate of selected QBAs may employ an in vitro model of rat liver microsomes (RLMs) with resulted QBA metabolites to be analyzed by means of liquid chromatography (LC) coupled to hybrid quadrupole time-of-flight (Q-TOF) mass spectrometry (MS).
Links
LH12176, research and development projectName: Benzofenanthridinové alkaloidy - studium účinků na celulární a molekulární úrovni (Acronym: KBF)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/0818/2012, interní kód MUName: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: BIOMET 2)
Investor: Masaryk University, Category A
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