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@article{1131973, author = {Stehlíková, Olga and Chovancová, Jana and Tichý, Boris and Krejčí, Marta and Brychtová, Yvona and Panovská, Anna and Skuhrová Francová, Hana and Burčková, Kateřina and Borský, Marek and Loja, Tomáš and Mayer, Jiří and Pospíšilová, Šárka and Doubek, Michael}, article_location = {Hoboken (USA)}, article_number = {2}, doi = {http://dx.doi.org/10.1111/ijlh.12149}, keywords = {CELL; CLL; FLUDARABINE; DIAGNOSIS; SURVIVAL; TRIAL}, language = {eng}, issn = {1751-5521}, journal = {International Journal of Laboratory Hematology}, title = {Detecting minimal residual disease in patients with chronic lymphocytic leukemia using 8-color flow cytometry protocol in routine hematological practice.}, url = {http://onlinelibrary.wiley.com/doi/10.1111/ijlh.12149/pdf}, volume = {36}, year = {2014} }
TY - JOUR ID - 1131973 AU - Stehlíková, Olga - Chovancová, Jana - Tichý, Boris - Krejčí, Marta - Brychtová, Yvona - Panovská, Anna - Skuhrová Francová, Hana - Burčková, Kateřina - Borský, Marek - Loja, Tomáš - Mayer, Jiří - Pospíšilová, Šárka - Doubek, Michael PY - 2014 TI - Detecting minimal residual disease in patients with chronic lymphocytic leukemia using 8-color flow cytometry protocol in routine hematological practice. JF - International Journal of Laboratory Hematology VL - 36 IS - 2 SP - 165-171 EP - 165-171 PB - Blackwell Scientific Publications SN - 17515521 KW - CELL KW - CLL KW - FLUDARABINE KW - DIAGNOSIS KW - SURVIVAL KW - TRIAL UR - http://onlinelibrary.wiley.com/doi/10.1111/ijlh.12149/pdf L2 - http://onlinelibrary.wiley.com/doi/10.1111/ijlh.12149/pdf N2 - Introduction Minimal residual disease (MRD) detection has become increasingly important for the assessment of therapy response in chronic lymphocytic leukemia (CLL). However, current MRD analysis methods, both molecular genetic and flow cytometric, are time-consuming and require experienced laboratory staff. Methods To reduce the demands of flow cytometric MRD detection in CLL, we have introduced a novel flow cytometric 8-color protocol. The MRD analysis results using this protocol were then compared with the commonly employed 4-color protocol and the molecular genetic (real-time quantitative allele-specific oligonucleotide IGH polymerase chain reaction; RQ-ASO IGH PCR) approach. Results Forty-two CLL patient samples were repeatedly analyzed after allogeneic stem cell transplantation (n=20) or after fludarabine-based therapy (n=22), and 100% concordance was found using both flow cytometric protocols. Furthermore, there was a strong correlation (r=0.94) between flow cytometric and RQ-ASO IGH PCR results in MRD detection. Conclusion Flow cytometry is less time-consuming, less financially demanding, and moreover, MRD assessment using our novel 8-color protocol is less complicated than the 4-color approach and molecular methods. ER -
STEHLÍKOVÁ, Olga, Jana CHOVANCOVÁ, Boris TICHÝ, Marta KREJČÍ, Yvona BRYCHTOVÁ, Anna PANOVSKÁ, Hana SKUHROVÁ FRANCOVÁ, Kateřina BURČKOVÁ, Marek BORSKÝ, Tomáš LOJA, Jiří MAYER, Šárka POSPÍŠILOVÁ and Michael DOUBEK. Detecting minimal residual disease in patients with chronic lymphocytic leukemia using 8-color flow cytometry protocol in routine hematological practice. \textit{International Journal of Laboratory Hematology}. Hoboken (USA): Blackwell Scientific Publications, vol.~36, No~2, p.~165-171. ISSN~1751-5521. doi:10.1111/ijlh.12149. 2014.
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