DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin.

FOŘTOVÁ, Andrea, Eva ŠEBESTOVÁ, Veronika ŠTĚPÁNKOVÁ, Táňa KOUDELÁKOVÁ, Lenka PÁLKOVÁ, Jiří DAMBORSKÝ and Radka CHALOUPKOVÁ. DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin. Biochimie. Elsevier, 2013, vol. 95, No 11, p. 2091-2096. ISSN 0300-9084. Available from: https://dx.doi.org/10.1016/j.biochi.2013.07.025.

Basic information

• Original name: DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin.
• Authors: FOŘTOVÁ, Andrea (203 Czech Republic, belonging to the institution), Eva ŠEBESTOVÁ (203 Czech Republic, belonging to the institution), Veronika ŠTĚPÁNKOVÁ (203 Czech Republic, belonging to the institution), Táňa KOUDELÁKOVÁ (203 Czech Republic, belonging to the institution), Lenka PÁLKOVÁ (203 Czech Republic, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution).
• Edition: Biochimie, Elsevier, 2013, 0300-9084.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.123
• RIV identification code: RIV/00216224:14310/13:00065807
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1016/j.biochi.2013.07.025
• UT WoS: 000325831000015
• Keywords in English: Haloalkane Dehalogenase
• Tags: AKR, rivok
• Tags: International impact, Reviewed

Abstract

Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards beta-brominated alkanes. Moreover, we identified other putative dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties.

Links

• ED0001/01/01, research and development project: Name: CETOCOEN
• GAP207/12/0775, research and development project: Name: Strukturně-funkční vztahy haloalkan dehalogenas

Investor: Czech Science Foundation

• IAA401630901, research and development project: Name: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami

Investor: Academy of Sciences of the Czech Republic, Evolution of substrate specificity in enzymes acting on xenobiotic compounds