DspA from Strongylocentrotus purpuratus: the First
Biochemically Characterized Haloalkane Dehalogenase of
Non-Microbial Origin.

J 2013

DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin.

FOŘTOVÁ, Andrea, Eva ŠEBESTOVÁ, Veronika ŠTĚPÁNKOVÁ, Táňa KOUDELÁKOVÁ, Lenka PÁLKOVÁ et. al.

Basic information

Original name

DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin.

Authors

FOŘTOVÁ, Andrea (203 Czech Republic, belonging to the institution), Eva ŠEBESTOVÁ (203 Czech Republic, belonging to the institution), Veronika ŠTĚPÁNKOVÁ (203 Czech Republic, belonging to the institution), Táňa KOUDELÁKOVÁ (203 Czech Republic, belonging to the institution), Lenka PÁLKOVÁ (203 Czech Republic, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Biochimie, Elsevier, 2013, 0300-9084

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.123

RIV identification code

RIV/00216224:14310/13:00065807

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.biochi.2013.07.025

UT WoS

000325831000015

Keywords in English

Haloalkane Dehalogenase

Abstract

V originále

Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards beta-brominated alkanes. Moreover, we identified other putative dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties.

Links

ED0001/01/01, research and development project

Name: CETOCOEN

GAP207/12/0775, research and development project

Name: Strukturně-funkční vztahy haloalkan dehalogenas

Investor: Czech Science Foundation

IAA401630901, research and development project

Name: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami

Investor: Academy of Sciences of the Czech Republic, Evolution of substrate specificity in enzymes acting on xenobiotic compounds

Citovat

FOŘTOVÁ, Andrea, Eva ŠEBESTOVÁ, Veronika ŠTĚPÁNKOVÁ, Táňa KOUDELÁKOVÁ, Lenka PÁLKOVÁ, Jiří DAMBORSKÝ and Radka CHALOUPKOVÁ. DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin. Biochimie. Elsevier, 2013, vol. 95, No 11, p. 2091-2096. ISSN 0300-9084. Available from: https://dx.doi.org/10.1016/j.biochi.2013.07.025.

@article{1131982,
   author = {Fořtová, Andrea and Šebestová, Eva and Štěpánková, Veronika and Koudeláková, Táňa and Pálková, Lenka and Damborský, Jiří and Chaloupková, Radka},
   article_number = {11},
   doi = {http://dx.doi.org/10.1016/j.biochi.2013.07.025},
   keywords = {Haloalkane Dehalogenase},
   language = {eng},
   issn = {0300-9084},
   journal = {Biochimie},
   title = {DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin.},
   volume = {95},
   year = {2013}
}

TY  - JOUR
ID  - 1131982
AU  - Fořtová, Andrea - Šebestová, Eva - Štěpánková, Veronika - Koudeláková, Táňa - Pálková, Lenka - Damborský, Jiří - Chaloupková, Radka
PY  - 2013
TI  - DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin.
JF  - Biochimie
VL  - 95
IS  - 11
SP  - 2091-2096
EP  - 2091-2096
PB  - Elsevier
SN  - 03009084
KW  - Haloalkane Dehalogenase
N2  - Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards beta-brominated alkanes. Moreover, we identified other putative dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties.
ER  -

FOŘTOVÁ, Andrea, Eva ŠEBESTOVÁ, Veronika ŠTĚPÁNKOVÁ, Táňa KOUDELÁKOVÁ, Lenka PÁLKOVÁ, Jiří DAMBORSKÝ and Radka CHALOUPKOVÁ. DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin. \textit{Biochimie}. Elsevier, 2013, vol.~95, No~11, p.~2091-2096. ISSN~0300-9084. Available from: https://dx.doi.org/10.1016/j.biochi.2013.07.025.

Detailed Information on Publication Record