FOŘTOVÁ, Andrea, Eva ŠEBESTOVÁ, Veronika ŠTĚPÁNKOVÁ, Táňa KOUDELÁKOVÁ, Lenka PÁLKOVÁ, Jiří DAMBORSKÝ and Radka CHALOUPKOVÁ. DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin. Biochimie. Elsevier, 2013, vol. 95, No 11, p. 2091-2096. ISSN 0300-9084. Available from: https://dx.doi.org/10.1016/j.biochi.2013.07.025. |
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@article{1131982, author = {Fořtová, Andrea and Šebestová, Eva and Štěpánková, Veronika and Koudeláková, Táňa and Pálková, Lenka and Damborský, Jiří and Chaloupková, Radka}, article_number = {11}, doi = {http://dx.doi.org/10.1016/j.biochi.2013.07.025}, keywords = {Haloalkane Dehalogenase}, language = {eng}, issn = {0300-9084}, journal = {Biochimie}, title = {DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin.}, volume = {95}, year = {2013} }
TY - JOUR ID - 1131982 AU - Fořtová, Andrea - Šebestová, Eva - Štěpánková, Veronika - Koudeláková, Táňa - Pálková, Lenka - Damborský, Jiří - Chaloupková, Radka PY - 2013 TI - DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin. JF - Biochimie VL - 95 IS - 11 SP - 2091-2096 EP - 2091-2096 PB - Elsevier SN - 03009084 KW - Haloalkane Dehalogenase N2 - Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards beta-brominated alkanes. Moreover, we identified other putative dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties. ER -
FOŘTOVÁ, Andrea, Eva ŠEBESTOVÁ, Veronika ŠTĚPÁNKOVÁ, Táňa KOUDELÁKOVÁ, Lenka PÁLKOVÁ, Jiří DAMBORSKÝ and Radka CHALOUPKOVÁ. DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin. \textit{Biochimie}. Elsevier, 2013, vol.~95, No~11, p.~2091-2096. ISSN~0300-9084. Available from: https://dx.doi.org/10.1016/j.biochi.2013.07.025.
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