PTP1B Is an Effector of Activin Signaling and Regulates Neural
Specification of Embryonic Stem Cells

J 2013

PTP1B Is an Effector of Activin Signaling and Regulates Neural Specification of Embryonic Stem Cells

MATULKA, Kamil, Hsuan-hwai LIN, Hana HŘÍBKOVÁ, Dafe UWANOGHO, Petr DVOŘÁK et. al.

Basic information

Original name

PTP1B Is an Effector of Activin Signaling and Regulates Neural Specification of Embryonic Stem Cells

Authors

MATULKA, Kamil (203 Czech Republic, belonging to the institution), Hsuan-hwai LIN (158 Taiwan), Hana HŘÍBKOVÁ (203 Czech Republic, belonging to the institution), Dafe UWANOGHO (826 United Kingdom of Great Britain and Northern Ireland), Petr DVOŘÁK (203 Czech Republic, guarantor, belonging to the institution) and Yuh-Man SUN (826 United Kingdom of Great Britain and Northern Ireland, belonging to the institution)

Edition

Cell stem cell, Cambridge, Cell Press, 2013, 1934-5909

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 22.151

RIV identification code

RIV/00216224:14110/13:00070445

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.stem.2013.09.016

UT WoS

000329571700013

Keywords in English

Activin/Nodal; protein tyrosine phosphatase 1B ; Activin/ALK4; p-ERK1/2 ;

Abstract

V originále

During embryogenesis, the Activin/Nodal pathway promotes the mesendodermal lineage and inhibits neural fate. The molecular mechanisms underlying this role of the Activin/Nodal pathway are not clear. In this study, we report a role for protein tyrosine phosphatase 1B (PTP1B) in Activin-mediated early fate decisions during ESC differentiation and show that PTP1B acts as an effector of the Activin pathway to specify mesendodermal or neural fate. We found that the Activin/ALK4 pathway directly recruits PTP1B and stimulates its release from the endoplasmic reticulum through ALK4-mediated cleavage. Subsequently, PTP1B suppresses p-ERK1/2 signaling to inhibit neural specification and promote mesendodermal commitment. These findings suggest that a noncanonical Activin signaling pathway functions in lineage specification of mouse and human embryonic stem cells.

Links

EE2.3.20.0011, research and development project

Name: Centrum výzkumu pluripotentních buněk a nestability genomu

MSM0021622430, plan (intention)

Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies

2SGA2878, interní kód MU

Name: Elucidating the role and molecular mechanism of Neuratin (NNAT) in neural induction (Acronym: NEURALDEVESTEM)

Investor: South-Moravian Region, Incoming grants

Citovat

MATULKA, Kamil, Hsuan-hwai LIN, Hana HŘÍBKOVÁ, Dafe UWANOGHO, Petr DVOŘÁK and Yuh-Man SUN. PTP1B Is an Effector of Activin Signaling and Regulates Neural Specification of Embryonic Stem Cells. Cell stem cell. Cambridge: Cell Press, 2013, vol. 13, No 6, p. 706-719. ISSN 1934-5909. Available from: https://dx.doi.org/10.1016/j.stem.2013.09.016.

@article{1135977,
   author = {Matulka, Kamil and Lin, Hsuanandhwai and Hříbková, Hana and Uwanogho, Dafe and Dvořák, Petr and Sun, YuhandMan},
   article_location = {Cambridge},
   article_number = {6},
   doi = {http://dx.doi.org/10.1016/j.stem.2013.09.016},
   keywords = {Activin/Nodal; protein tyrosine phosphatase 1B ; Activin/ALK4; p-ERK1/2 ;},
   language = {eng},
   issn = {1934-5909},
   journal = {Cell stem cell},
   note = {2SGA2878 - projekt SoMoPro Jihomoravského kraje - bohužel nejde dle RIV kombinovat finance územních správních celků a VZ apod.},
   title = {PTP1B Is an Effector of Activin Signaling and Regulates Neural Specification of Embryonic Stem Cells},
   url = {http://www.sciencedirect.com/science/article/pii/S1934590913004463},
   volume = {13},
   year = {2013}
}

TY  - JOUR
ID  - 1135977
AU  - Matulka, Kamil - Lin, Hsuan-hwai - Hříbková, Hana - Uwanogho, Dafe - Dvořák, Petr - Sun, Yuh-Man
PY  - 2013
TI  - PTP1B Is an Effector of Activin Signaling and Regulates Neural Specification of Embryonic Stem Cells
JF  - Cell stem cell
VL  - 13
IS  - 6
SP  - 706-719
EP  - 706-719
PB  - Cell Press
SN  - 19345909
N1  - 2SGA2878 - projekt SoMoPro Jihomoravského kraje - bohužel nejde dle RIV kombinovat finance územních správních celků a VZ apod.
KW  - Activin/Nodal
KW  - protein tyrosine phosphatase 1B
KW  - Activin/ALK4
KW  - p-ERK1/2 ;
UR  - http://www.sciencedirect.com/science/article/pii/S1934590913004463
N2  - During embryogenesis, the Activin/Nodal pathway promotes the mesendodermal lineage and inhibits neural fate. The molecular mechanisms underlying this role of the Activin/Nodal pathway are not clear. In this study, we report a role for protein tyrosine phosphatase 1B (PTP1B) in Activin-mediated early fate decisions during ESC differentiation and show that PTP1B acts as an effector of the Activin pathway to specify mesendodermal or neural fate. We found that the Activin/ALK4 pathway directly recruits PTP1B and stimulates its release from the endoplasmic reticulum through ALK4-mediated cleavage. Subsequently, PTP1B suppresses p-ERK1/2 signaling to inhibit neural specification and promote mesendodermal commitment. These findings suggest that a noncanonical Activin signaling pathway functions in lineage specification of mouse and human embryonic stem cells.
ER  -

MATULKA, Kamil, Hsuan-hwai LIN, Hana HŘÍBKOVÁ, Dafe UWANOGHO, Petr DVOŘÁK and Yuh-Man SUN. PTP1B Is an Effector of Activin Signaling and Regulates Neural Specification of Embryonic Stem Cells. \textit{Cell stem cell}. Cambridge: Cell Press, 2013, vol.~13, No~6, p.~706-719. ISSN~1934-5909. Available from: https://dx.doi.org/10.1016/j.stem.2013.09.016.

Detailed Information on Publication Record