Cisplatin-related differences in antioxidant system, apoptosis and cell cyclle in prostate cancer cell lines

GUMULEC, Jaromír a Michal MASAŘÍK. Cisplatin-related differences in antioxidant system, apoptosis and cell cyclle in prostate cancer cell lines. In XIII. Pracovní setkání fyzikálních chemiků a elektrochemiků. 2013.

Základní údaje

• Originální název: Cisplatin-related differences in antioxidant system, apoptosis and cell cyclle in prostate cancer cell lines
• Autoři: GUMULEC, Jaromír a Michal MASAŘÍK.
• Vydání: XIII. Pracovní setkání fyzikálních chemiků a elektrochemiků, 2013.

Další údaje

• Typ výsledku: Konferenční abstrakt
• Utajení: není předmětem státního či obchodního tajemství

Anotace

This study integrates the results of antioxidant and flow-cytometric assays in order to characterize prostate cancer cell lines (PNT1A, 22Rv1, PC-3) under cis-diamminedichloroplatinum (cisplatin) treatment. Cell cycle, activity of antioxidant system related proteins (SOD, GPx, GR, metallothionein), and free radical scavenging capacity assays (Free radicals (FR), Ferric Reducing Antioxidant Power (FRAP), ABTS) were analyzed with respect to the rising concentrations (0–150 microM) and different length of cisplatin treatment (12–72 h). Non-functional-p53 PC-3 cell line shows in contrast to PNT1A and 22Rv1 no cisplatin-induced cell cycle arrest. All cell lines show increasing level of free radical scavenging activity by ABTS, FRAP and FR assays in time- and dose-dependent manner (r > 0.76 at p < 0.001 for ABTS, FRAP and FR at p < 0.001). PC-3 shows the most increased (p < 0.05) level of free radical scavenging activity by ABTS and FR methods in comparison with other cell lines. These data suggest an increased ability of later stages of prostate cancer tumorous cells to cope with oxidative stress conditions.