FARKA, Zdeněk, David KOVÁŘ and Petr SKLÁDAL. Piezoelectric biosensor coupled to cyclone air sampler for detection of microorganisms. Chemické listy. Praha: Česká společnost chemická, 2013, vol. 107, s3, p. s302-s304, 3 pp. ISSN 0009-2770.
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Basic information
Original name Piezoelectric biosensor coupled to cyclone air sampler for detection of microorganisms
Authors FARKA, Zdeněk (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution).
Edition Chemické listy, Praha, Česká společnost chemická, 2013, 0009-2770.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW zacina na 14. strance v souboru
Impact factor Impact factor: 0.196
RIV identification code RIV/00216224:14310/13:00070523
Organization unit Faculty of Science
UT WoS 000328730800008
Keywords (in Czech) bioaerosol; biosensor; imunosensor; QCM
Keywords in English Bioaerosol; Biosensor; Immunosensor; QCM
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Petr Skládal, CSc., učo 2202. Changed: 25/3/2014 11:27.
Abstract
Even though microorganisms are common part of our lives, some of them are dangerous and must be monitored. The traditional microbiological methods are reliable but slow. In some cases, namely in military, key requirement is fast detection which can be provided by biosensors. In this work, the piezoelectric biosensors providing fast, specific and cheap way of detection are described. Three strains of E. coli (BL21, DH5 alpha and K-12) were chosen as safe model microbes and the detection using the developed piezoelectric biosensor was carried out (LOD 10^6 CFU mL-1, total analysis time 20 min). Bioaerosol chamber was constructed to allow safe dissemination of microbes in air and experiments with bioaerosol. The generated bioaerosol was sampled using the cyclone air sampler and analyzed with the on-line coupled piezoelectric biosensor. The levels of 10^6 CFU L-1 of E. coli in air were successfully detected in less than 26 min which demonstrates possible application of this system for rapid screening of microorganisms in the air.
Abstract (in Czech)
Piezoelectrický imunosensor pro detekci kmenů Escherichia coli (BL21, DH5 alpha, K-12) ve formě bioaerosolu.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
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