Immobilized Enzyme Reactor for On-Line Studies of Drug Metabolism Mediated by Cytochrome P450 2C9 Isoform

SCHEJBAL, Jan, Roman ŘEMÍNEK, Lukáš ZEMAN, Marta ZEISBERGEROVÁ and Zdeněk GLATZ. Immobilized Enzyme Reactor for On-Line Studies of Drug Metabolism Mediated by Cytochrome P450 2C9 Isoform. Chemické listy. Praha: Česká společnost chemická, 2013, vol. 107, S3, p. "S441" - "S443", 3 pp. ISSN 0009-2770.

Basic information

• Original name: Immobilized Enzyme Reactor for On-Line Studies of Drug Metabolism Mediated by Cytochrome P450 2C9 Isoform
• Name in Czech: Immobilizovaný enzymový reaktor pro on-line studie metabolismu léčiv zprostředkovaného cytochromem P450 2C9
• Authors: SCHEJBAL, Jan (203 Czech Republic, belonging to the institution), Roman ŘEMÍNEK (203 Czech Republic, belonging to the institution), Lukáš ZEMAN (203 Czech Republic, belonging to the institution), Marta ZEISBERGEROVÁ (203 Czech Republic, belonging to the institution) and Zdeněk GLATZ (203 Czech Republic, guarantor).
• Edition: Chemické listy, Praha, Česká společnost chemická, 2013, 0009-2770.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 0.196
• RIV identification code: RIV/00216224:14310/13:00066625
• Organization unit: Faculty of Science
• UT WoS: 000328730800064
• Keywords (in Czech): Kapilární elektroforéza; metabolismus léčiv; CYP2C9; diklofenak; imobilizace, IMER
• Keywords in English: Capillary Electrophoresis; Drug Metabolism; CYP2C9; Diclofenac; Immobilization; IMER
• Tags: AKR, CE, CE methods, CYP2C9, diclofenac, drug metabolism, in-capillary reaction, rivok
• Tags: International impact, Reviewed

Abstract

Drug biotransformation mediated by cytochrome P450 enzymes (CYP) is a pivotal factor in the early developmental stages of new drugs. Metabolism appraisal of every hit thus constitutes the inseparable part of ADME/tox screenings. Enzyme immobilization represents a useful approach in this field because of CYP recycling and simplification of sequential analyses by providing protein-free samples. A new method for immobilization of therapeutically important cytochrome P450 2C9 isoform (CYP2C9) on magnetic microparticles SiMAG Carboxyl is thus presented.

Abstract (in Czech)

Byla vyvinuta metoda pro imobilizaci terapeuticky významného cytochromu P450 2C9 na magnetických mikročásticích SiMAG-Carboxyl. Po optimalizaci parametrů imobilizace a separačních podmínek je možné systém využít k on-line inhibičním studiím tohoto enzymu.

Links

• EE2.3.20.0182, research and development project: Name: Vytvoření multidisciplinárního výzkumného a vzdělávacího centra bioanalytických technologií
• GAP206/10/0057, research and development project: Name: Miniaturizovaný systém pro "on-line" studie metabolismu léčiv založený na kapilární elektroforéze

Investor: Czech Science Foundation, Miniaturized on-line drug metabolism system based on capillary electrophoresis