Microfluidic chip coupled with modified paramagnetic particles
for sarcosine isolation in urine

J 2013

Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine

ZITKA, Ondrej, Natalia CERNEI, Zbynek HEGER, Miroslav MATOUSEK, Pavel KOPEL et. al.

Basic information

Original name

Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine

Authors

ZITKA, Ondrej (203 Czech Republic), Natalia CERNEI (203 Czech Republic), Zbynek HEGER (203 Czech Republic), Miroslav MATOUSEK (203 Czech Republic), Pavel KOPEL (203 Czech Republic), Jindrich KYNICKY (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution), Rene KIZEK (203 Czech Republic) and Vojtech ADAM (203 Czech Republic)

Edition

Electrophoresis, Hoboken, Wiley-Blackwell, 2013, 0173-0835

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.161

RIV identification code

RIV/00216224:14110/13:00070631

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/elps.201300114

UT WoS

000327590900006

Keywords in English

Ion exchange chromatography; Magnetic nanoparticles; Microfluidic separation; Prostate cancer; Surface modification

Abstract

V originále

Carcinoma of prostate (CaP) is the second most frequent malignant tumor occurring in men in Europe. Currently there is discussion on a wide range of potential CaP markers. One of them-nonprotein amino acid sarcosine, also known as N-methylglycine was chosen as a challenge for the development of microfluidic system with isolation by modified paramagnetic microparticles. Therefore, the aim of this study was to design a low-cost, simple, and rapid microfluidic system based on sarcosine isolation with modified paramagnetic microparticles and subsequent analysis on the ion exchange LC. We modified Dowex microparticles with Fe2O3 nanoparticles. Our paramagnetic microparticles were able to establish the binding with sarcosine. Moreover, we designed microfluidic device for sarcosine determination. Analysis of samples was carried out with LOD of 1 uM of a sarcosine that is sufficient because it is similar to concentrations of a sarcosine observed in the CaP patients.

Citovat

ZITKA, Ondrej, Natalia CERNEI, Zbynek HEGER, Miroslav MATOUSEK, Pavel KOPEL, Jindrich KYNICKY, Michal MASAŘÍK, Rene KIZEK and Vojtech ADAM. Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine. Electrophoresis. Hoboken: Wiley-Blackwell, 2013, vol. 34, No 18, p. 2639-2647. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.201300114.

@article{1137575,
   author = {Zitka, Ondrej and Cernei, Natalia and Heger, Zbynek and Matousek, Miroslav and Kopel, Pavel and Kynicky, Jindrich and Masařík, Michal and Kizek, Rene and Adam, Vojtech},
   article_location = {Hoboken},
   article_number = {18},
   doi = {http://dx.doi.org/10.1002/elps.201300114},
   keywords = {Ion exchange chromatography; Magnetic nanoparticles; Microfluidic separation; Prostate cancer; Surface modification},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine},
   volume = {34},
   year = {2013}
}

TY  - JOUR
ID  - 1137575
AU  - Zitka, Ondrej - Cernei, Natalia - Heger, Zbynek - Matousek, Miroslav - Kopel, Pavel - Kynicky, Jindrich - Masařík, Michal - Kizek, Rene - Adam, Vojtech
PY  - 2013
TI  - Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine
JF  - Electrophoresis
VL  - 34
IS  - 18
SP  - 2639-2647
EP  - 2639-2647
PB  - Wiley-Blackwell
SN  - 01730835
KW  - Ion exchange chromatography
KW  - Magnetic nanoparticles
KW  - Microfluidic separation
KW  - Prostate cancer
KW  - Surface modification
N2  - Carcinoma of prostate (CaP) is the second most frequent malignant tumor occurring in men in Europe. Currently there is discussion on a wide range of potential CaP markers. One of them-nonprotein amino acid sarcosine, also known as N-methylglycine was chosen as a challenge for the development of microfluidic system with isolation by modified paramagnetic microparticles. Therefore, the aim of this study was to design a low-cost, simple, and rapid microfluidic system based on sarcosine isolation with modified paramagnetic microparticles and subsequent analysis on the ion exchange LC. We modified Dowex microparticles with Fe2O3 nanoparticles. Our paramagnetic microparticles were able to establish the binding with sarcosine. Moreover, we designed microfluidic device for sarcosine determination. Analysis of samples was carried out with LOD of 1 uM of a sarcosine that is sufficient because it is similar to concentrations of a sarcosine observed in the CaP patients.
ER  -

ZITKA, Ondrej, Natalia CERNEI, Zbynek HEGER, Miroslav MATOUSEK, Pavel KOPEL, Jindrich KYNICKY, Michal MASAŘÍK, Rene KIZEK and Vojtech ADAM. Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine. \textit{Electrophoresis}. Hoboken: Wiley-Blackwell, 2013, vol.~34, No~18, p.~2639-2647. ISSN~0173-0835. Available from: https://dx.doi.org/10.1002/elps.201300114.

Detailed Information on Publication Record