ZITKA, Ondrej, Natalia CERNEI, Zbynek HEGER, Miroslav MATOUSEK, Pavel KOPEL, Jindrich KYNICKY, Michal MASAŘÍK, Rene KIZEK and Vojtech ADAM. Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine. Electrophoresis. Hoboken: Wiley-Blackwell, 2013, vol. 34, No 18, p. 2639-2647. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.201300114. |
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@article{1137575, author = {Zitka, Ondrej and Cernei, Natalia and Heger, Zbynek and Matousek, Miroslav and Kopel, Pavel and Kynicky, Jindrich and Masařík, Michal and Kizek, Rene and Adam, Vojtech}, article_location = {Hoboken}, article_number = {18}, doi = {http://dx.doi.org/10.1002/elps.201300114}, keywords = {Ion exchange chromatography; Magnetic nanoparticles; Microfluidic separation; Prostate cancer; Surface modification}, language = {eng}, issn = {0173-0835}, journal = {Electrophoresis}, title = {Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine}, volume = {34}, year = {2013} }
TY - JOUR ID - 1137575 AU - Zitka, Ondrej - Cernei, Natalia - Heger, Zbynek - Matousek, Miroslav - Kopel, Pavel - Kynicky, Jindrich - Masařík, Michal - Kizek, Rene - Adam, Vojtech PY - 2013 TI - Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine JF - Electrophoresis VL - 34 IS - 18 SP - 2639-2647 EP - 2639-2647 PB - Wiley-Blackwell SN - 01730835 KW - Ion exchange chromatography KW - Magnetic nanoparticles KW - Microfluidic separation KW - Prostate cancer KW - Surface modification N2 - Carcinoma of prostate (CaP) is the second most frequent malignant tumor occurring in men in Europe. Currently there is discussion on a wide range of potential CaP markers. One of them-nonprotein amino acid sarcosine, also known as N-methylglycine was chosen as a challenge for the development of microfluidic system with isolation by modified paramagnetic microparticles. Therefore, the aim of this study was to design a low-cost, simple, and rapid microfluidic system based on sarcosine isolation with modified paramagnetic microparticles and subsequent analysis on the ion exchange LC. We modified Dowex microparticles with Fe2O3 nanoparticles. Our paramagnetic microparticles were able to establish the binding with sarcosine. Moreover, we designed microfluidic device for sarcosine determination. Analysis of samples was carried out with LOD of 1 uM of a sarcosine that is sufficient because it is similar to concentrations of a sarcosine observed in the CaP patients. ER -
ZITKA, Ondrej, Natalia CERNEI, Zbynek HEGER, Miroslav MATOUSEK, Pavel KOPEL, Jindrich KYNICKY, Michal MASAŘÍK, Rene KIZEK and Vojtech ADAM. Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine. \textit{Electrophoresis}. Hoboken: Wiley-Blackwell, 2013, vol.~34, No~18, p.~2639-2647. ISSN~0173-0835. Available from: https://dx.doi.org/10.1002/elps.201300114.
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