Inflammatory profiling of the satellite glial cells in the dorsal root ganglia of rat experimental neuropathic pain models

DUBOVÝ, Petr, Václav BRÁZDA, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ and Lucie KUBÍČKOVÁ. Inflammatory profiling of the satellite glial cells in the dorsal root ganglia of rat experimental neuropathic pain models. In The XI European Meeting on Glial Cell Function in Health and Disease, Berlin. 2013. ISSN 0894-1491.

Basic information

• Original name: Inflammatory profiling of the satellite glial cells in the dorsal root ganglia of rat experimental neuropathic pain models
• Name in Czech: Zánětová charakteristika satelitních gliových buněk spinálních ganglií v experimentálních modelech neuropatické bolesti
• Authors: DUBOVÝ, Petr, Václav BRÁZDA, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ and Lucie KUBÍČKOVÁ.
• Edition: The XI European Meeting on Glial Cell Function in Health and Disease, Berlin, 2013.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30000 3. Medical and Health Sciences
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 5.466
• Organization unit: Central European Institute of Technology
• ISSN: 0894-1491
• UT WoS: 000320408400539
• Keywords (in Czech): satelitní gliové buňky, cytokiny, neuropatická bolest
• Keywords in English: satellite glial cells, cytokines, neuropathic pain

Abstract

The satellite glial cells (SGC) envelope the primary sensory neurons of the dorsal root ganglia (DRG) and react intensely to various types of nerve injury that induce neuropathic pain. Unilateral chronic constriction injury (CCI, n=64) and spare nerve injury (SNI, n=32) of the rat sciatic nerve well as i.p. application of paclitaxel (PAC, n=12) were used as experimental models of neuropathic pain (NPP). NPP induction was tested by withdrawal threshold of mechanoallodynia and thermal hyperalgesia. Expression of TNFa, IL-6 and their receptor and signaling (TNFR1, TNFR2, IL-6R, gp130, STAT3) proteins and mRNA were investigated bilaterally by immunohistochemistry and in situ hybridization in both lumbar (L4-5) and cervical (C7-8) DRG 1, 3, 7 and 14 days from treatment. The SGC were identified by colocalization with GFAP or GS. Our results demonstrate significant inflammatory activation of the neurons and their SGC not only in DRG associated but also non-associated with injured nerve. A distinct expression of cytokines and their receptors was identified in SGC surrounding large-sized DRG neurons. Significant inflammatory reactions of SGC were found in all DRG of PAC-treated rats. Moreover, inflammatory activation SGC was also observed in DRG of sham-operated rats indicating other kinds of trigger than traumatic nerve injury. The nerve injury and PAC-treatment also triggered SOCS3 expression in SGC to control STAT3 activation. Inflammatory activation of SGC significantly contributes to ectopic activation of the DRG neurons not only associated with injured nerve, and is involved in NPP induction.

Abstract (in Czech)

Výsledky prokázaly zánětovou aktivaci satelitních gliových buněk v modelech neuropatické bolesti.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• 286154, interní kód MU: Name: SYLICA - Synergies of Life and Material Sciences to Create a New Future (Acronym: SYLICA)

Investor: European Union, Capacities