The effect of oyster mushroom beta-1.3/1.6-D-glucan and
oxytetracycline antibiotic on biometrical, haematological,
biochemical, and immunological indices, and histopathological
changes in common carp (Cyprinus carpio L.)

J 2013

The effect of oyster mushroom beta-1.3/1.6-D-glucan and oxytetracycline antibiotic on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in common carp (Cyprinus carpio L.)

DOBŠÍKOVÁ, Radka, Jana BLAHOVÁ, Ivana MIKULÍKOVÁ, Helena MODRÁ, Eva PRÁŠKOVÁ et. al.

Basic information

Original name

The effect of oyster mushroom beta-1.3/1.6-D-glucan and oxytetracycline antibiotic on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in common carp (Cyprinus carpio L.)

Authors

DOBŠÍKOVÁ, Radka (203 Czech Republic), Jana BLAHOVÁ (203 Czech Republic), Ivana MIKULÍKOVÁ (203 Czech Republic), Helena MODRÁ (203 Czech Republic), Eva PRÁŠKOVÁ (203 Czech Republic), Zdeňka SVOBODOVÁ (203 Czech Republic), Mišo ŠKOŘRČ (203 Czech Republic), Jiří JARKOVSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Andrzej-Krzysztof SIWICKI (616 Poland)

Edition

Fish & shellfish immunology, London, Academic Press, 2013, 1050-4648

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 3.034

RIV identification code

RIV/00216224:14110/13:00070723

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.fsi.2013.09.006

UT WoS

000330158800014

Keywords in English

Blood and plasma indices; Chemiluminescence; Condition factor; Fish; Immunoglobulins

Abstract

V originále

The aim of the study was to evaluate the effect of micronized B-1.3/1.6-D-glucan (BG) derived from the oyster mushroom Pleurotus ostreatus Hiratake and tetracycline antibiotic oxytetracycline (OTC) on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in tissues of one- to two-year-old common carp (Cyprinus carpio L.). The fish tested were divided into five experimental groups and one control. Carp in the control group were fed commercial carp feed pellets. Fish in the five experimental groups were fed the same pellets supplemented with either OTC, a combination of OTC and BG, or BG as follows: 75mgoxytetracyclinekg-1 bw (OTC group), 75mgoxytetracyclinekg-1 bw and 0.5% B-glucan (OTC+0.5% BG group), 75mgoxytetracyclinekg-1 bw and 2.0% B-glucan (OTC+2.0% BG group), 0.5% B-glucan (0.5% BG group), and 2.0% B-glucan (2.0% BG group). OTC- and BG-supplemented diets and the control diet were administered to experimental and control carp for 50 days (i.e. samplings 1-3, the exposure period); for the following 14 days, fish were fed only control feed pellets with no OTC or BG supplementation (i.e. sampling 4, the recovery period). Blood and tissue samples were collected both during, and at the end of the study. No significant changes in biometrical indices (i.e. total length, standard length, total weight, hepatosomatic and spleen somatic index, and Fulton's condition factor) were found in experimental carp compared to control in any sampling. In haematological indices, significant changes were found only in sampling 2, in which shifts in PCV (P<0.01), Hb (P<0.01), and WBC (P<0.01), and in the counts of lymphocytes (P<0.01), monocytes (P<0.01), and neutrophil granulocytes-segments (P<0.05) were revealed. As for biochemical profiling, plasma concentrations of glucose, albumins, cholesterol, natrium, and chlorides (all P<0.01), and total proteins, lactate, phosphorus, and potassium (all P<0.05) as well as the catalytic activity of ALP (P<0.05) were altered in common carp. A significant change in induced (opsonizedzymosan particles, OZP) chemiluminescence (P<0.05) in sampling 3 and no shifts in serum immunoglobulins concentration were found in the immunological analysis. Histopathological examination of skin, gills, liver, spleen, and cranial and caudal kidneys revealed no obvious specific changes in any tissue analysed. The use of B-glucans in clinically healthy aquaculture remains an issue. Nevertheless, their use in breeding endangered by stress stimuli, infectious disease, or adverse environmental factors is defensible.

Citovat

DOBŠÍKOVÁ, Radka, Jana BLAHOVÁ, Ivana MIKULÍKOVÁ, Helena MODRÁ, Eva PRÁŠKOVÁ, Zdeňka SVOBODOVÁ, Mišo ŠKOŘRČ, Jiří JARKOVSKÝ and Andrzej-Krzysztof SIWICKI. The effect of oyster mushroom beta-1.3/1.6-D-glucan and oxytetracycline antibiotic on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in common carp (Cyprinus carpio L.). Fish & shellfish immunology. London: Academic Press, 2013, vol. 35, No 6, p. 1813-1823. ISSN 1050-4648. Available from: https://dx.doi.org/10.1016/j.fsi.2013.09.006.

@article{1138451,
   author = {Dobšíková, Radka and Blahová, Jana and Mikulíková, Ivana and Modrá, Helena and Prášková, Eva and Svobodová, Zdeňka and Škořrč, Mišo and Jarkovský, Jiří and Siwicki, AndrzejandKrzysztof},
   article_location = {London},
   article_number = {6},
   doi = {http://dx.doi.org/10.1016/j.fsi.2013.09.006},
   keywords = {Blood and plasma indices; Chemiluminescence; Condition factor; Fish; Immunoglobulins},
   language = {eng},
   issn = {1050-4648},
   journal = {Fish & shellfish immunology},
   title = {The effect of oyster mushroom beta-1.3/1.6-D-glucan and oxytetracycline antibiotic on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in common carp (Cyprinus carpio L.)},
   url = {https://www.sciencedirect.com/science/article/pii/S1050464813007511},
   volume = {35},
   year = {2013}
}

TY  - JOUR
ID  - 1138451
AU  - Dobšíková, Radka - Blahová, Jana - Mikulíková, Ivana - Modrá, Helena - Prášková, Eva - Svobodová, Zdeňka - Škořrč, Mišo - Jarkovský, Jiří - Siwicki, Andrzej-Krzysztof
PY  - 2013
TI  - The effect of oyster mushroom beta-1.3/1.6-D-glucan and oxytetracycline antibiotic on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in common carp (Cyprinus carpio L.)
JF  - Fish & shellfish immunology
VL  - 35
IS  - 6
SP  - 1813-1823
EP  - 1813-1823
PB  - Academic Press
SN  - 10504648
KW  - Blood and plasma indices
KW  - Chemiluminescence
KW  - Condition factor
KW  - Fish
KW  - Immunoglobulins
UR  - https://www.sciencedirect.com/science/article/pii/S1050464813007511
N2  - The aim of the study was to evaluate the effect of micronized B-1.3/1.6-D-glucan (BG) derived from the oyster mushroom Pleurotus ostreatus Hiratake and tetracycline antibiotic oxytetracycline (OTC) on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in tissues of one- to two-year-old common carp (Cyprinus carpio L.). The fish tested were divided into five experimental groups and one control. Carp in the control group were fed commercial carp feed pellets. Fish in the five experimental groups were fed the same pellets supplemented with either OTC, a combination of OTC and BG, or BG as follows: 75mgoxytetracyclinekg-1 bw (OTC group), 75mgoxytetracyclinekg-1 bw and 0.5% B-glucan (OTC+0.5% BG group), 75mgoxytetracyclinekg-1 bw and 2.0% B-glucan (OTC+2.0% BG group), 0.5% B-glucan (0.5% BG group), and 2.0% B-glucan (2.0% BG group). OTC- and BG-supplemented diets and the control diet were administered to experimental and control carp for 50 days (i.e. samplings 1-3, the exposure period); for the following 14 days, fish were fed only control feed pellets with no OTC or BG supplementation (i.e. sampling 4, the recovery period). Blood and tissue samples were collected both during, and at the end of the study. No significant changes in biometrical indices (i.e. total length, standard length, total weight, hepatosomatic and spleen somatic index, and Fulton's condition factor) were found in experimental carp compared to control in any sampling. In haematological indices, significant changes were found only in sampling 2, in which shifts in PCV (P<0.01), Hb (P<0.01), and WBC (P<0.01), and in the counts of lymphocytes (P<0.01), monocytes (P<0.01), and neutrophil granulocytes-segments (P<0.05) were revealed. As for biochemical profiling, plasma concentrations of glucose, albumins, cholesterol, natrium, and chlorides (all P<0.01), and total proteins, lactate, phosphorus, and potassium (all P<0.05) as well as the catalytic activity of ALP (P<0.05) were altered in common carp. A significant change in induced (opsonizedzymosan particles, OZP) chemiluminescence (P<0.05) in sampling 3 and no shifts in serum immunoglobulins concentration were found in the immunological analysis. Histopathological examination of skin, gills, liver, spleen, and cranial and caudal kidneys revealed no obvious specific changes in any tissue analysed. The use of B-glucans in clinically healthy aquaculture remains an issue. Nevertheless, their use in breeding endangered by stress stimuli, infectious disease, or adverse environmental factors is defensible.
ER  -

DOBŠÍKOVÁ, Radka, Jana BLAHOVÁ, Ivana MIKULÍKOVÁ, Helena MODRÁ, Eva PRÁŠKOVÁ, Zdeňka SVOBODOVÁ, Mišo ŠKOŘRČ, Jiří JARKOVSKÝ and Andrzej-Krzysztof SIWICKI. The effect of oyster mushroom beta-1.3/1.6-D-glucan and oxytetracycline antibiotic on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in common carp (Cyprinus carpio L.). \textit{Fish \&{} shellfish immunology}. London: Academic Press, 2013, vol.~35, No~6, p.~1813-1823. ISSN~1050-4648. Available from: https://dx.doi.org/10.1016/j.fsi.2013.09.006.

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