HORKÁ, Marie, Pavel KARÁSEK, Jiří ŠALPLACHTA, Filip RŮŽIČKA, Marie VYKYDALOVÁ, Anna KUBESOVÁ, Vladimír DRÁB, Michal ROTH a Karel ŠLAIS. Capillary isoelectric focusing of probiotic bacteria from cow's milk in tapered fused silica capillary with off-line matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification. Analytica Chimica Acta. AMSTERDAM: ELSEVIER SCIENCE BV, 2013, roč. 788, 25 July 2013, s. 193-199. ISSN 0003-2670. doi:10.1016/j.aca.2013.05.059.
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Základní údaje
Originální název Capillary isoelectric focusing of probiotic bacteria from cow's milk in tapered fused silica capillary with off-line matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification
Autoři HORKÁ, Marie (203 Česká republika), Pavel KARÁSEK (203 Česká republika), Jiří ŠALPLACHTA (203 Česká republika), Filip RŮŽIČKA (203 Česká republika, garant, domácí), Marie VYKYDALOVÁ (203 Česká republika), Anna KUBESOVÁ (203 Česká republika), Vladimír DRÁB (203 Česká republika), Michal ROTH (203 Česká republika) a Karel ŠLAIS (203 Česká republika).
Vydání Analytica Chimica Acta, AMSTERDAM, ELSEVIER SCIENCE BV, 2013, 0003-2670.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10600 1.6 Biological sciences
Stát vydavatele Nizozemsko
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 4.517
Kód RIV RIV/00216224:14110/13:00070727
Organizační jednotka Lékařská fakulta
Doi http://dx.doi.org/10.1016/j.aca.2013.05.059
UT WoS 000321497700027
Klíčová slova anglicky Capillary isoelectric focusing; Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; Tapered fused silica capillary; Lactic acid bacteria; Milk
Štítky EL OK, podil
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Ing. Mgr. Věra Pospíšilíková, učo 9005. Změněno: 29. 3. 2014 23:24.
Anotace
In this study, combination of capillary isoelectric focusing (CIEF) in tapered fused silica (FS) capillary with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is presented as an efficient approach for unambiguous identification of probiotic bacteria in real sample. For this purpose, bacteria within genus Lactobacillus were selected as model bioanalytes and cow's milk was selected as a biological sample. CIEF analysis of both the cultivated bacteria and the bacteria in the milk was optimized and isoelectric points characterizing the examined bacteria were subsequently determined independently of the bacterial sample origin. The use of tapered FS capillary significantly enhanced the separation capacity and efficiency of the CIEF analyses performed. In addition, the cell number injected into the tapered FS capillary was quantified and an excellent linearity of the calibration curves was achieved which enabled quantitative analysis of the bacteria by CIEF with UV detection. The minimum detectable number of bacterial cells was 2 x 10(6) mL(-1). Finally, cow's milk spiked with the selected bacterium was analyzed by CIEF in tapered FS capillary, the focused and detected bacterial cells were collected from the capillary, deposited onto the cultivation medium, and identified using MALDI-TOF MS afterward. Our results have revealed that the proposed procedure can be advantageously used for unambiguous identification of probiotic bacteria in a real sample.
VytisknoutZobrazeno: 18. 5. 2022 08:57