| VYKYDALOVÁ, Marie, Marie HORKÁ, Filip RŮŽIČKA, Filip DUŠA, Dana MORAVCOVÁ, Vladislav KAHLE a Karel ŠLAIS. Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter. Analytica Chimica Acta. AMSTERDAM: ELSEVIER SCIENCE BV, 2014, roč. 812, FEB, s. 243-249. ISSN 0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2013.12.032. |
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@article{1138634,
author = {Vykydalová, Marie and Horká, Marie and Růžička, Filip and Duša, Filip and Moravcová, Dana and Kahle, Vladislav and Šlais, Karel},
article_location = {AMSTERDAM},
article_number = {FEB},
doi = {http://dx.doi.org/10.1016/j.aca.2013.12.032},
keywords = {Candida parapsilosis group; biofilm; micropreparative solution isoelectric focusing; high-performance liquid chromatography; capillary isoelectric focusing; sonicate from vascular catheter},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter},
url = {http://www.sciencedirect.com/science/article/pii/S000326701301581X},
volume = {812},
year = {2014}
}
TY - JOUR
ID - 1138634
AU - Vykydalová, Marie - Horká, Marie - Růžička, Filip - Duša, Filip - Moravcová, Dana - Kahle, Vladislav - Šlais, Karel
PY - 2014
TI - Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter
JF - Analytica Chimica Acta
VL - 812
IS - FEB
SP - 243-249
EP - 243-249
PB - ELSEVIER SCIENCE BV
SN - 00032670
KW - Candida parapsilosis group
KW - biofilm
KW - micropreparative solution isoelectric focusing
KW - high-performance liquid chromatography
KW - capillary isoelectric focusing
KW - sonicate from vascular catheter
UR - http://www.sciencedirect.com/science/article/pii/S000326701301581X
L2 - http://www.sciencedirect.com/science/article/pii/S000326701301581X
N2 - This study utilizes the high-performance liquid chromatography technique in combination with the new micropreparative solution isoelectric focusing fractionation on non-woven fabric strip for the characterization and differentiation of biofilm-positive and biofilm-negative forms of Candida parapsilosis sensu stricto on the basis of the changes in the composition of their cell-surface. Treatment of yeasts by boiling in distilled water relased surface substances from yeasts cells. Consequently, the optimized procedure has been used for fast identification of the highly pathogenic biofilm-positive Candida parapsilosis group in real clinical material – sonicate from vascular catheters. Moreover, the capillary isoelectric focusing was used as supporting and control technique. Obtained results suggest that this new method can be used to distinguish between biofilm-positive and negative forms of Candida parapsilosis sensu stricto.
ER -
VYKYDALOVÁ, Marie, Marie HORKÁ, Filip RŮŽIČKA, Filip DUŠA, Dana MORAVCOVÁ, Vladislav KAHLE a Karel ŠLAIS. Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter. \textit{Analytica Chimica Acta}. AMSTERDAM: ELSEVIER SCIENCE BV, 2014, roč.~812, FEB, s.~243-249. ISSN~0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2013.12.032.
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