| BREJCHA, Martin, Martina STOKLASOVÁ, Yvona BRYCHTOVÁ, Anna PANOVSKÁ, Kristina ŠTĚPANOVSKÁ, Gabriela VAŇKOVÁ, Karla PLEVOVÁ, Alexandra OLTOVÁ, Kateřina HORKÁ, Šárka POSPÍŠILOVÁ, Jiří MAYER a Michael DOUBEK. Clonal evolution in chronic lymphocytic leukemia detected by fluorescence in situ hybridization and conventional cytogenetics after stimulation with CpG oligonucleotides and interleukin-2: A prospective analysis. Leukemia Research. Oxford: Pergamon Elsevier, 2014, roč. 38, č. 2, s. 170-175. ISSN 0145-2126. Dostupné z: https://dx.doi.org/10.1016/j.leukres.2013.10.019. |
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@article{1138698,
author = {Brejcha, Martin and Stoklasová, Martina and Brychtová, Yvona and Panovská, Anna and Štěpanovská, Kristina and Vaňková, Gabriela and Plevová, Karla and Oltová, Alexandra and Horká, Kateřina and Pospíšilová, Šárka and Mayer, Jiří and Doubek, Michael},
article_location = {Oxford},
article_number = {2},
doi = {http://dx.doi.org/10.1016/j.leukres.2013.10.019},
keywords = {Chronic lymphocytic leukemia; Clonal evolution; CpG oligonucleotides; Cytogenetics; Fluorescence in situ hybridization; Interleukin-2},
language = {eng},
issn = {0145-2126},
journal = {Leukemia Research},
title = {Clonal evolution in chronic lymphocytic leukemia detected by fluorescence in situ hybridization and conventional cytogenetics after stimulation with CpG oligonucleotides and interleukin-2: A prospective analysis.},
url = {http://ac.els-cdn.com/S0145212613003743/1-s2.0-S0145212613003743-main.pdf?_tid=94685418-c7f7-11e4-a9b3-00000aacb362&acdnat=1426082868_d96dbfd19537a9a8ff20a6f2dd3b991a},
volume = {38},
year = {2014}
}
TY - JOUR
ID - 1138698
AU - Brejcha, Martin - Stoklasová, Martina - Brychtová, Yvona - Panovská, Anna - Štěpanovská, Kristina - Vaňková, Gabriela - Plevová, Karla - Oltová, Alexandra - Horká, Kateřina - Pospíšilová, Šárka - Mayer, Jiří - Doubek, Michael
PY - 2014
TI - Clonal evolution in chronic lymphocytic leukemia detected by fluorescence in situ hybridization and conventional cytogenetics after stimulation with CpG oligonucleotides and interleukin-2: A prospective analysis.
JF - Leukemia Research
VL - 38
IS - 2
SP - 170-175
EP - 170-175
PB - Pergamon Elsevier
SN - 01452126
KW - Chronic lymphocytic leukemia
KW - Clonal evolution
KW - CpG oligonucleotides
KW - Cytogenetics
KW - Fluorescence in situ hybridization
KW - Interleukin-2
UR - http://ac.els-cdn.com/S0145212613003743/1-s2.0-S0145212613003743-main.pdf?_tid=94685418-c7f7-11e4-a9b3-00000aacb362&acdnat=1426082868_d96dbfd19537a9a8ff20a6f2dd3b991a
L2 - http://ac.els-cdn.com/S0145212613003743/1-s2.0-S0145212613003743-main.pdf?_tid=94685418-c7f7-11e4-a9b3-00000aacb362&acdnat=1426082868_d96dbfd19537a9a8ff20a6f2dd3b991a
N2 - Chronic lymphocytic leukemia (CLL) patients may acquire new chromosome abnormalities during the course of their disease. Clonal evolution (CE) has been detected by conventional chromosome banding (CBA), several groups also confirmed CE with fluorescence in situ hybridization (FISH). At present, there are minimal prospective data on CE frequency determined using a combination of both methods. Therefore, the aim of our study was to prospectively assess CE frequency using a combination of FISH and CBA after stimulation with CpG oligonucleotides and interleukin-2. Between 2008 and 2012, we enrolled 140 patients with previously untreated CLL in a prospective trial evaluating CE using FISH and CBA after stimulation. Patients provided baseline and regular follow-up peripheral blood samples for testing. There was a median of 3 cytogenetic examinations (using both methods) per patient. CE was detected in 15.7% (22/140) of patients using FISH, in 28.6% (40/140) using CBA, and in 34.3% (48/140) of patients by combining both methods. Poor-prognosis CE (new deletion 17p, new deletion 11q or new complex karyotype) was detected in 15% (21/140) of patients and was significantly associated with previous CLL treatment (p=0.013). CBA provides more complex information about cytogenetic abnormalities in CLL patients than FISH and confirms that many patients can acquire new abnormalities during the course of their disease in a relatively short time period.
ER -
BREJCHA, Martin, Martina STOKLASOVÁ, Yvona BRYCHTOVÁ, Anna PANOVSKÁ, Kristina ŠTĚPANOVSKÁ, Gabriela VAŇKOVÁ, Karla PLEVOVÁ, Alexandra OLTOVÁ, Kateřina HORKÁ, Šárka POSPÍŠILOVÁ, Jiří MAYER a Michael DOUBEK. Clonal evolution in chronic lymphocytic leukemia detected by fluorescence in situ hybridization and conventional cytogenetics after stimulation with CpG oligonucleotides and interleukin-2: A prospective analysis. \textit{Leukemia Research}. Oxford: Pergamon Elsevier, 2014, roč.~38, č.~2, s.~170-175. ISSN~0145-2126. Dostupné z: https://dx.doi.org/10.1016/j.leukres.2013.10.019.
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