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@article{1138867,
author = {Štěpánková, Veronika and Damborský, Jiří and Chaloupková, Radka},
article_number = {6},
doi = {http://dx.doi.org/10.1002/biot.201200378},
keywords = {Haloalkane Dehalogenases},
language = {eng},
issn = {1860-6768},
journal = {Biotechnology Journal},
title = {Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases},
volume = {8},
year = {2013}
}
TY - JOUR
ID - 1138867
AU - Štěpánková, Veronika - Damborský, Jiří - Chaloupková, Radka
PY - 2013
TI - Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases
JF - Biotechnology Journal
VL - 8
IS - 6
SP - 719-729
EP - 719-729
SN - 18606768
KW - Haloalkane Dehalogenases
N2 - Haloalkane dehalogenases are microbial enzymes with a wide range of biotechnological applications. The use of organic co-solvents to solubilize their hydrophobic substrates is often necessary. In order to choose the most compatible co-solvent, the effects of fourteen co-solvents on activity, stability and enantioselectivity of three model enzymes, DbjA, DhaA and LinB, were evaluated. All co-solvents caused at high concentration loss of activity and conformational changes. The highest inactivation was induced by tetrahydrofuran, while more hydrophilic co-solvents, such as ethylene glycol and dimethyl sulfoxide, were more tolerated. The effects of co-solvents at low concentration were different for each enzyme-solvent pair. The increase in DbjA activity was induced by the majority of organic co-solvents tested, while activities of DhaA and LinB decreased at comparable concentrations of the same co-solvent. Moreover, high increase of DbjA enantioselectivity was observed. Ethylene glycol and 1,4-dioxane were shown to have the most positive impact on the enantioselectivity. The favourable influence of the co-solvents on both activity and enantioselectivity makes DbjA suitable for biocatalytic applications. This study represents the first investigation of the effects of organic co-solvents on the catalytic performance of haloalkane dehalogenases and will pave the way for their broader use in industrial processes.
ER -
ŠTĚPÁNKOVÁ, Veronika, Jiří DAMBORSKÝ and Radka CHALOUPKOVÁ. Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases. \textit{Biotechnology Journal}. 2013, vol.~8, No~6, p.~719-729. ISSN~1860-6768. Available from: https://dx.doi.org/10.1002/biot.201200378.
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