Sphingobium czechense sp nov., isolated from a hexachlorocyclohexane dump site

NIHARIKA, Neha, Hana MOSKALÍKOVÁ, Jasvinder KAUR, Fazlurrahman KHAN, Miroslava SEDLÁČKOVÁ, Aleš HAMPL, Jiří DAMBORSKÝ, Zbyněk PROKOP and Rup LAL. Sphingobium czechense sp nov., isolated from a hexachlorocyclohexane dump site. International Journal of Systematic and Evolutionary Microbiology. SOC GENERAL MICROBIOLOGY, 2013, vol. 63, Part 2, p. 723-728. ISSN 1466-5026. Available from: https://dx.doi.org/10.1099/ijs.0.039396-0.

Basic information

• Original name: Sphingobium czechense sp nov., isolated from a hexachlorocyclohexane dump site
• Authors: NIHARIKA, Neha (356 India), Hana MOSKALÍKOVÁ (203 Czech Republic), Jasvinder KAUR (356 India), Fazlurrahman KHAN (356 India), Miroslava SEDLÁČKOVÁ (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution), Zbyněk PROKOP (203 Czech Republic, belonging to the institution) and Rup LAL (356 India).
• Edition: International Journal of Systematic and Evolutionary Microbiology, SOC GENERAL MICROBIOLOGY, 2013, 1466-5026.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 2.798
• RIV identification code: RIV/00216224:14310/13:00065824
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1099/ijs.0.039396-0
• UT WoS: 000317170400052
• Keywords in English: LIN GENES; SPHINGOMONAS; IDENTIFICATION; DEGRADATION; ACID; MICROORGANISMS; PHYLOGENY; BACTERIA; STRAIN; SOILS
• Tags: AKR, podil, rivok
• Tags: International impact, Reviewed

Abstract

A yellow pigmented bacterial strain, designated LL01T, was isolated from hexachlorocyclohexane (HCH)-contaminated soil in Spolana Neratovice, former Czech producer of Lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL01T occupied a distinct phylogenetic position in the Sphingobium cluster, showing highest similarity with Sphingobium rhizovicinum CC-FH12-1T (98.5%). The DNA G+C content of strain LL01T was 66.1 mol%. The predominant respiratory pigment ubiquonone Q10 is present. The polar lipid profile of the strain LL01T also corresponded to those reported for other Sphingobium species (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipids), supporting its identification as member of the genus Sphingobium. Spermidine was the major polyamine observed in the cell. The results obtained after DNA-DNA hybridization, biochemical and physiological tests clearly distinguished it from closely related species of the genus Sphingobium. Therefore, strain LL01T represents a novel species of the genus Sphingobium for which the name Sphingobium czechense LL01T sp. nov. is proposed (=CCM 7979T=DSM 25410T).

Links

• ED0001/01/01, research and development project: Name: CETOCOEN
• GAP202/10/1435, research and development project: Name: Analýza a vizualizace proteinových struktur

Investor: Czech Science Foundation, Analysis and Visualization of Protein Structures

• GA203/08/0114, research and development project: Name: Specifické iontové efekty pro proteiny v roztocích a podobné biologicky relevantní systémy.

Investor: Czech Science Foundation, Specific ion effects for proteins in solutions and related biologically relevant systems

• IAA401630901, research and development project: Name: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami

Investor: Academy of Sciences of the Czech Republic, Evolution of substrate specificity in enzymes acting on xenobiotic compounds