Intracellular distribution of the deltaNp73 protein isoform in
medulloblastoma cells: a study with newly generated rabbit
polyclonal antibodies

J 2013

Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

VESELSKÁ, Renata, Jakub NERADIL, Marta NEKULOVÁ, Lucia DOBRUCKÁ, Bořivoj VOJTĚŠEK et. al.

Basic information

Original name

Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

Authors

VESELSKÁ, Renata (203 Czech Republic, guarantor, belonging to the institution), Jakub NERADIL (203 Czech Republic, belonging to the institution), Marta NEKULOVÁ (203 Czech Republic, belonging to the institution), Lucia DOBRUCKÁ (703 Slovakia, belonging to the institution), Bořivoj VOJTĚŠEK (203 Czech Republic), Jaroslav ŠTĚRBA (203 Czech Republic, belonging to the institution) and Karel ZITTERBART (203 Czech Republic, belonging to the institution)

Edition

Histology and Histopathology, 2013, 0213-3911

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Spain

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.236

RIV identification code

RIV/00216224:14310/13:00065656

Organization unit

Faculty of Science

UT WoS

000320710900011

Keywords in English

medulloblastoma;deltaNp73;immunocytochemistry;immunoblotting;transmission electron microscopy

Abstract

V originále

This study is focused on the intracellular localization of Delta Np73 in medulloblastoma cell lines. Due to a lack of suitable anti-Delta Np73 antibodies, we developed two new rabbit polyclonal antibodies, Delta Np7326 and Delta Np73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the Delta Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the Delta Np73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the Delta Np73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with Delta Np73 and golgin-97, we showed the co-localization of the Delta Np73 isoform with the Golgi apparatus.

Links

NS10218, research and development project

Name: Role izoforem proteinu p73 v patogenezi meduloblastomu: imunocytochemická a microarray studie

Investor: Ministry of Health of the CR

Citovat

VESELSKÁ, Renata, Jakub NERADIL, Marta NEKULOVÁ, Lucia DOBRUCKÁ, Bořivoj VOJTĚŠEK, Jaroslav ŠTĚRBA and Karel ZITTERBART. Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies. Histology and Histopathology. 2013, vol. 28, No 7, p. 913-924. ISSN 0213-3911.

@article{1139223,
   author = {Veselská, Renata and Neradil, Jakub and Nekulová, Marta and Dobrucká, Lucia and Vojtěšek, Bořivoj and Štěrba, Jaroslav and Zitterbart, Karel},
   article_number = {7},
   keywords = {medulloblastoma;deltaNp73;immunocytochemistry;immunoblotting;transmission electron microscopy},
   language = {eng},
   issn = {0213-3911},
   journal = {Histology and Histopathology},
   title = {Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies},
   url = {http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm},
   volume = {28},
   year = {2013}
}

TY  - JOUR
ID  - 1139223
AU  - Veselská, Renata - Neradil, Jakub - Nekulová, Marta - Dobrucká, Lucia - Vojtěšek, Bořivoj - Štěrba, Jaroslav - Zitterbart, Karel
PY  - 2013
TI  - Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies
JF  - Histology and Histopathology
VL  - 28
IS  - 7
SP  - 913-924
EP  - 913-924
SN  - 02133911
KW  - medulloblastoma;deltaNp73;immunocytochemistry;immunoblotting;transmission electron microscopy
UR  - http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm
L2  - http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm
N2  - This study is focused on the intracellular localization of Delta Np73 in medulloblastoma cell lines. Due to a lack of suitable anti-Delta Np73 antibodies, we developed two new rabbit polyclonal antibodies, Delta Np7326 and Delta Np73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the Delta Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the Delta Np73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the Delta Np73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with Delta Np73 and golgin-97, we showed the co-localization of the Delta Np73 isoform with the Golgi apparatus.
ER  -

VESELSKÁ, Renata, Jakub NERADIL, Marta NEKULOVÁ, Lucia DOBRUCKÁ, Bořivoj VOJTĚŠEK, Jaroslav ŠTĚRBA and Karel ZITTERBART. Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies. \textit{Histology and Histopathology}. 2013, vol.~28, No~7, p.~913-924. ISSN~0213-3911.

Detailed Information on Publication Record