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@article{1139223, author = {Veselská, Renata and Neradil, Jakub and Nekulová, Marta and Dobrucká, Lucia and Vojtěšek, Bořivoj and Štěrba, Jaroslav and Zitterbart, Karel}, article_number = {7}, keywords = {medulloblastoma;deltaNp73;immunocytochemistry;immunoblotting;transmission electron microscopy}, language = {eng}, issn = {0213-3911}, journal = {Histology and Histopathology}, title = {Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies}, url = {http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm}, volume = {28}, year = {2013} }
TY - JOUR ID - 1139223 AU - Veselská, Renata - Neradil, Jakub - Nekulová, Marta - Dobrucká, Lucia - Vojtěšek, Bořivoj - Štěrba, Jaroslav - Zitterbart, Karel PY - 2013 TI - Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies JF - Histology and Histopathology VL - 28 IS - 7 SP - 913-924 EP - 913-924 SN - 02133911 KW - medulloblastoma;deltaNp73;immunocytochemistry;immunoblotting;transmission electron microscopy UR - http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm L2 - http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm N2 - This study is focused on the intracellular localization of Delta Np73 in medulloblastoma cell lines. Due to a lack of suitable anti-Delta Np73 antibodies, we developed two new rabbit polyclonal antibodies, Delta Np7326 and Delta Np73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the Delta Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the Delta Np73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the Delta Np73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with Delta Np73 and golgin-97, we showed the co-localization of the Delta Np73 isoform with the Golgi apparatus. ER -
VESELSKÁ, Renata, Jakub NERADIL, Marta NEKULOVÁ, Lucia DOBRUCKÁ, Bořivoj VOJTĚŠEK, Jaroslav ŠTĚRBA and Karel ZITTERBART. Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies. \textit{Histology and Histopathology}. 2013, vol.~28, No~7, p.~913-924. ISSN~0213-3911.
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