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@article{1139332,
author = {Bidmanová, Šárka and Damborský, Jiří and Prokop, Zbyněk},
article_number = {2},
keywords = {Haloalkane Dehalogenase},
language = {eng},
issn = {2324-9099},
journal = {Journal of Biocatalysis & Biotransformation},
title = {Immobilization of Haloalkane Dehalogenase LinB from Sphingobium japonicum UT26 for Biotechnological Applications},
volume = {2},
year = {2013}
}
TY - JOUR
ID - 1139332
AU - Bidmanová, Šárka - Damborský, Jiří - Prokop, Zbyněk
PY - 2013
TI - Immobilization of Haloalkane Dehalogenase LinB from Sphingobium japonicum UT26 for Biotechnological Applications
JF - Journal of Biocatalysis & Biotransformation
VL - 2
IS - 2
SP - 1-7
EP - 1-7
SN - 23249099
KW - Haloalkane Dehalogenase
N2 - Haloalkane dehalogenases are enzymes capable of converting a broad range of aliphatic halogenated compounds to corresponding alcohols. These dehalogenase-based biotransformations are attractive for various biological processes, e.g. biocatalysis, bioremediation and detoxification, which often require protein immobilization. Different immobilization techniques, including (i) cross-linking using glutaraldehyde, dextran polyaldehyde, disuccinimidyl suberate and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, (ii) formation of cross-linked enzyme aggregates, and (iii) entrapment methods using organically modified sol-gel ORMOCER and polyvinyl alcohol particles, were systematically investigated for immobilization of selected haloalkane dehalogenase LinB from Sphingobium japonicum UT26.
ER -
BIDMANOVÁ, Šárka, Jiří DAMBORSKÝ a Zbyněk PROKOP. Immobilization of Haloalkane Dehalogenase LinB from Sphingobium japonicum UT26 for Biotechnological Applications. \textit{Journal of Biocatalysis \&{} Biotransformation}. 2013, roč.~2, č.~2, s.~1-7. ISSN~2324-9099.
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