Detailed Information on Publication Record
2014
Forced aggregation and defined factors allow highly uniform-sized embryoid bodies and functional cardiomyocytes from human embryonic and induced pluripotent stem cells
PEŠL, Martin, Ivana AĆIMOVIĆ, Jan PŘIBYL, Renata HÉŽOVÁ, Aleksandra VILOTIĆ et. al.Basic information
Original name
Forced aggregation and defined factors allow highly uniform-sized embryoid bodies and functional cardiomyocytes from human embryonic and induced pluripotent stem cells
Authors
PEŠL, Martin (203 Czech Republic, guarantor, belonging to the institution), Ivana AĆIMOVIĆ (688 Serbia, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Renata HÉŽOVÁ (203 Czech Republic, belonging to the institution), Aleksandra VILOTIĆ (688 Serbia, belonging to the institution), Jeremy FAUCONNIER (250 France), Jan VRBSKÝ (203 Czech Republic, belonging to the institution), Peter KRUZLIAK (203 Czech Republic), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Tomáš KÁRA (203 Czech Republic), Vladimír ROTREKL (203 Czech Republic, belonging to the institution), Alain LACAMPAGNE (250 France), Petr DVOŘÁK (203 Czech Republic, belonging to the institution) and Albano MELI (250 France, belonging to the institution)
Edition
Heart Vessels, Japan, Springer Japan, 2014, 0910-8327
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Japan
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.065
RIV identification code
RIV/00216224:14110/14:00074888
Organization unit
Faculty of Medicine
UT WoS
000345145200014
Keywords (in Czech)
human pluripotent stem cell embryoid body differentiation cardiomyocyte calcium
Keywords in English
human pluripotent stem cell embryoid body differentiation cardiomyocyte calcium
Tags
Tags
International impact, Reviewed
Změněno: 15/1/2015 12:06, Ing. Mgr. Věra Pospíšilíková
Abstract
V originále
In vitro human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) can differentiate into functional cardiomyocytes (CMs). Protocols for cardiac differentiation of hESCs and hiPSCs include formation of the three-dimensional cell aggregates called embryoid bodies (EBs). The traditional suspension method for EB formation from clumps of cells results in an EB population heterogeneous in size and shape. In this study we show that forced aggregation of a defined number of single cells on AggreWell plates gives a high number of homogeneous EBs that can be efficiently differentiated into functional CMs by application of defined growth factors in the media. For cardiac differentiation, we used three hESC lines and one hiPSC line. Our contracting EBs and the resulting CMs express cardiac markers, namely myosin heavy chain alpha and beta, cardiac ryanodine receptor/calcium release channel, and cardiac troponin T, shown by real-time polymerase chain reaction and immunocytochemistry. Using Ca2+ imaging and atomic force microscopy, we demonstrate the functionality of RyR2 to release Ca2+ from the sarcoplasmic reticulum as well as reliability in contractile and beating properties of hESC-EBs and hiPSC-EBs upon the stimulation or inhibition of the beta-adrenergic pathway.
Links
| ED1.1.00/02.0068, research and development project |
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| EE2.3.20.0011, research and development project |
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| MSM0021622430, plan (intention) |
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| 7AMB13FR011, research and development project |
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