| PEŠL, Martin, Ivana AĆIMOVIĆ, Jan PŘIBYL, Renata HÉŽOVÁ, Aleksandra VILOTIĆ, Jeremy FAUCONNIER, Jan VRBSKÝ, Peter KRUZLIAK, Petr SKLÁDAL, Tomáš KÁRA, Vladimír ROTREKL, Alain LACAMPAGNE, Petr DVOŘÁK a Albano MELI. Forced aggregation and defined factors allow highly uniform-sized embryoid bodies and functional cardiomyocytes from human embryonic and induced pluripotent stem cells. Heart Vessels. Japan: Springer Japan, 2014, roč. 29, č. 6, s. 834-846. ISSN 0910-8327. Dostupné z: https://dx.doi.org/10.1007/s00380-013-0436-9. |
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@article{1139396,
author = {Pešl, Martin and Aćimović, Ivana and Přibyl, Jan and Héžová, Renata and Vilotić, Aleksandra and Fauconnier, Jeremy and Vrbský, Jan and Kruzliak, Peter and Skládal, Petr and Kára, Tomáš and Rotrekl, Vladimír and Lacampagne, Alain and Dvořák, Petr and Meli, Albano},
article_location = {Japan},
article_number = {6},
doi = {http://dx.doi.org/10.1007/s00380-013-0436-9},
keywords = {human pluripotent stem cell embryoid body differentiation cardiomyocyte calcium},
language = {eng},
issn = {0910-8327},
journal = {Heart Vessels},
title = {Forced aggregation and defined factors allow highly uniform-sized embryoid bodies and functional cardiomyocytes from human embryonic and induced pluripotent stem cells},
url = {http://link.springer.com/article/10.1007/s00380-013-0436-9#},
volume = {29},
year = {2014}
}
TY - JOUR
ID - 1139396
AU - Pešl, Martin - Aćimović, Ivana - Přibyl, Jan - Héžová, Renata - Vilotić, Aleksandra - Fauconnier, Jeremy - Vrbský, Jan - Kruzliak, Peter - Skládal, Petr - Kára, Tomáš - Rotrekl, Vladimír - Lacampagne, Alain - Dvořák, Petr - Meli, Albano
PY - 2014
TI - Forced aggregation and defined factors allow highly uniform-sized embryoid bodies and functional cardiomyocytes from human embryonic and induced pluripotent stem cells
JF - Heart Vessels
VL - 29
IS - 6
SP - 834-846
EP - 834-846
PB - Springer Japan
SN - 09108327
KW - human pluripotent stem cell embryoid body differentiation cardiomyocyte calcium
UR - http://link.springer.com/article/10.1007/s00380-013-0436-9#
N2 - In vitro human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) can differentiate into functional cardiomyocytes (CMs). Protocols for cardiac differentiation of hESCs and hiPSCs include formation of the three-dimensional cell aggregates called embryoid bodies (EBs). The traditional suspension method for EB formation from clumps of cells results in an EB population heterogeneous in size and shape. In this study we show that forced aggregation of a defined number of single cells on AggreWell plates gives a high number of homogeneous EBs that can be efficiently differentiated into functional CMs by application of defined growth factors in the media. For cardiac differentiation, we used three hESC lines and one hiPSC line. Our contracting EBs and the resulting CMs express cardiac markers, namely myosin heavy chain alpha and beta, cardiac ryanodine receptor/calcium release channel, and cardiac troponin T, shown by real-time polymerase chain reaction and immunocytochemistry. Using Ca2+ imaging and atomic force microscopy, we demonstrate the functionality of RyR2 to release Ca2+ from the sarcoplasmic reticulum as well as reliability in contractile and beating properties of hESC-EBs and hiPSC-EBs upon the stimulation or inhibition of the beta-adrenergic pathway.
ER -
PEŠL, Martin, Ivana A$\backslash$'CIMOVI$\backslash$'C, Jan PŘIBYL, Renata HÉŽOVÁ, Aleksandra VILOTI$\backslash$'C, Jeremy FAUCONNIER, Jan VRBSKÝ, Peter KRUZLIAK, Petr SKLÁDAL, Tomáš KÁRA, Vladimír ROTREKL, Alain LACAMPAGNE, Petr DVOŘÁK a Albano MELI. Forced aggregation and defined factors allow highly uniform-sized embryoid bodies and functional cardiomyocytes from human embryonic and induced pluripotent stem cells. \textit{Heart Vessels}. Japan: Springer Japan, 2014, roč.~29, č.~6, s.~834-846. ISSN~0910-8327. Dostupné z: https://dx.doi.org/10.1007/s00380-013-0436-9.
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