J 2013

A new liquid chromatography-tandem mass spectrometry method using atmospheric pressure photo ionization for the simultaneous determination of azaarenes and azaarones in Dutch river sediments

BRULÍK, Jan, Zdeněk ŠIMEK and Pim DE VOOGT

Basic information

Original name

A new liquid chromatography-tandem mass spectrometry method using atmospheric pressure photo ionization for the simultaneous determination of azaarenes and azaarones in Dutch river sediments

Authors

BRULÍK, Jan (203 Czech Republic, guarantor, belonging to the institution), Zdeněk ŠIMEK (203 Czech Republic, belonging to the institution) and Pim DE VOOGT (528 Netherlands)

Edition

Journal of Chromatography A, AMSTERDAM, ELSEVIER SCIENCE BV, 2013, 0021-9673

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.258

RIV identification code

RIV/00216224:14310/13:00071055

Organization unit

Faculty of Science

DOI

UT WoS

000319848000005

Keywords in English

Azaarenes; Azaarones; PAH; APPI; LC-MS/MS; Sedimets

Tags

Tags

International impact, Reviewed
Změněno: 27/3/2014 16:42, Ing. Andrea Mikešková

Abstract

V originále

A new method for the analysis of azaarenes and their degradation products (azaarones) was developed, optimized and validated using liquid chromatography coupled with atmospheric pressure photo ionization tandem mass spectrometric detection (LC-APPI/MS/MS). Seventeen compounds including 4 PAHs (naphthalene, anthracene, phenanthrene, benz[a]anthracene), 7 azaarenes (quinoline, acridine, phenanthridine, 5,6-benzoquinoline and 7,8-benzoquinoline, benzo[a]acridine, benzo[c]acridine), and 6 azaarones (2-OH-quinoline, 4-OH-quinoline, 5-OH-quinoline, 6-OH-quinoline, 9(10H)-acridone, 6(5H)phenanthridinone) were analyzed in sediment samples from Dutch rivers. All compounds were analyzed simultaneously in multi reaction monitoring (MRM) mode. Soxhlet extraction was used for the extraction of analytes from sediments. The limits of quantification of azaarenes and azaarones varied from 0.21 to 1.12 mu g/l and from 0.23 to 1.58 mu g/l, respectively. The limits of quantification for PAHs varied from 32 to 769 mu g/l. Matrix-independent recoveries of sediment samples were in the range 85-110%; matrix-dependent recoveries were in the range 73-148%, respectively. The method was tested on real sediment samples and the results were compared with a previous study in which GC/MS/MS was used for the simultaneous measurement of azaarenes and azaarones. 4-, 5- and 6-OH-quinolines and naphthalene, anthracene and phenanthrene were not present or below detection limits in some samples. All other analytes were present in samples in the concentration range 0.2-1200 ng/g (dw). To our knowledge, this is the first report showing the possibility of measurement non-polar polyaromatic hydrocarbons together with polar azaarenes and their degradation products azaarones simultaneously with sufficient sensitivity and accuracy using LC/MS/MS.

Links

ED0001/01/01, research and development project
Name: CETOCOEN
LM2011028, research and development project
Name: RECETOX ? Národní infrastruktura pro výzkum toxických látek v prostředí
Investor: Ministry of Education, Youth and Sports of the CR
MSM0021622412, plan (intention)
Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)