The isolation and characterization of lipopolysaccharides from
Microcystis aeruginosa, a prominent toxic water bloom forming
cyanobacteria

J 2013

The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria

BLÁHOVÁ, Lucie, Ondřej ADAMOVSKÝ, Lukáš KUBALA, Lenka SINDLEROVA SVIHALKOVA, Radka ZOUNKOVÁ et. al.

Basic information

Original name

The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria

Authors

BLÁHOVÁ, Lucie (203 Czech Republic, belonging to the institution), Ondřej ADAMOVSKÝ (203 Czech Republic, belonging to the institution), Lukáš KUBALA (203 Czech Republic), Lenka SINDLEROVA SVIHALKOVA (203 Czech Republic), Radka ZOUNKOVÁ (203 Czech Republic, belonging to the institution) and Luděk BLÁHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Toxicon, OXFORD, Elsevier Science, 2013, 0041-0101

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.581

RIV identification code

RIV/00216224:14310/13:00066817

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.toxicon.2013.10.011

UT WoS

000328658600023

Keywords in English

Endotoxin; Cyanobacteria; Water bloom; Lipopolysaccharide; Microcystis

Abstract

V originále

Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide, yet serious gaps exist in understanding their complex toxic effects, including the role of lipopolysaccharides (LPS). The present comparative study focused on the levels and biological activities of LPS isolated from Microcystis aeruginosa, which is one of the most globally distributed toxic species. Using hot phenol extraction, LPS was isolated from 3 laboratory cultures and 11 natural water blooms. It formed 0.2-0.7% of the original dry biomass of the cyanobacteria, based on gravimetry. Additional analyses by commercial anti-LPS ELISA were correlated with gravimetry but showed concentrations that were about 7-times lower, which indicated either impurities in isolated LPS or the poor cross-reactivity of the antibodies used. LPS isolates from M. aeruginosa were potent pyrogens in the traditional Limulus amebocyte lysate (LAL)-test, but comparison with the PyroGene test demonstrated the limited selectivity of LAL with several interferences. The determined pyrogenicity (endotoxin units, EU) ranged from very low values in laboratory cultures (less than 0.003 up to 0.008-EU per 100 pg LPS) to higher values in complex bloom samples (0.01-0.078 EU per 100 pg of LPS), which suggested the role of bloom-associated bacteria in the overall effects. Potent pro-inflammatory effects of the studied LPS from both cultures and bloom samples were observed in a highly-relevant ex vivo human blood model by studying reactive oxygen species production in phagocytes as well as increased productions of interleukin 8, IL-8, and tumor necrosis factor alpha, TNF-alpha. LPS from M aeruginosa seem to modulate several pathways involved in the regulation of both innate immunity and specific responses.

Links

ED0001/01/01, research and development project

Name: CETOCOEN

GP13-27695P, research and development project

Name: Metabolity sinic jako možné modulátory imunitního systému (Acronym: immunotox)

Investor: Czech Science Foundation

Citovat

BLÁHOVÁ, Lucie, Ondřej ADAMOVSKÝ, Lukáš KUBALA, Lenka SINDLEROVA SVIHALKOVA, Radka ZOUNKOVÁ and Luděk BLÁHA. The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria. Toxicon. OXFORD: Elsevier Science, 2013, vol. 76, No 2013, p. 187-196. ISSN 0041-0101. Available from: https://dx.doi.org/10.1016/j.toxicon.2013.10.011.

@article{1161548,
   author = {Bláhová, Lucie and Adamovský, Ondřej and Kubala, Lukáš and Sindlerova Svihalkova, Lenka and Zounková, Radka and Bláha, Luděk},
   article_location = {OXFORD},
   article_number = {2013},
   doi = {http://dx.doi.org/10.1016/j.toxicon.2013.10.011},
   keywords = {Endotoxin; Cyanobacteria; Water bloom; Lipopolysaccharide; Microcystis},
   language = {eng},
   issn = {0041-0101},
   journal = {Toxicon},
   title = {The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria},
   volume = {76},
   year = {2013}
}

TY  - JOUR
ID  - 1161548
AU  - Bláhová, Lucie - Adamovský, Ondřej - Kubala, Lukáš - Sindlerova Svihalkova, Lenka - Zounková, Radka - Bláha, Luděk
PY  - 2013
TI  - The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria
JF  - Toxicon
VL  - 76
IS  - 2013
SP  - 187-196
EP  - 187-196
PB  - Elsevier Science
SN  - 00410101
KW  - Endotoxin
KW  - Cyanobacteria
KW  - Water bloom
KW  - Lipopolysaccharide
KW  - Microcystis
N2  - Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide, yet serious gaps exist in understanding their complex toxic effects, including the role of lipopolysaccharides (LPS). The present comparative study focused on the levels and biological activities of LPS isolated from Microcystis aeruginosa, which is one of the most globally distributed toxic species. Using hot phenol extraction, LPS was isolated from 3 laboratory cultures and 11 natural water blooms. It formed 0.2-0.7% of the original dry biomass of the cyanobacteria, based on gravimetry. Additional analyses by commercial anti-LPS ELISA were correlated with gravimetry but showed concentrations that were about 7-times lower, which indicated either impurities in isolated LPS or the poor cross-reactivity of the antibodies used. LPS isolates from M. aeruginosa were potent pyrogens in the traditional Limulus amebocyte lysate (LAL)-test, but comparison with the PyroGene test demonstrated the limited selectivity of LAL with several interferences. The determined pyrogenicity (endotoxin units, EU) ranged from very low values in laboratory cultures (less than 0.003 up to 0.008-EU per 100 pg LPS) to higher values in complex bloom samples (0.01-0.078 EU per 100 pg of LPS), which suggested the role of bloom-associated bacteria in the overall effects. Potent pro-inflammatory effects of the studied LPS from both cultures and bloom samples were observed in a highly-relevant ex vivo human blood model by studying reactive oxygen species production in phagocytes as well as increased productions of interleukin 8, IL-8, and tumor necrosis factor alpha, TNF-alpha. LPS from M aeruginosa seem to modulate several pathways involved in the regulation of both innate immunity and specific responses.
ER  -

BLÁHOVÁ, Lucie, Ondřej ADAMOVSKÝ, Lukáš KUBALA, Lenka SINDLEROVA SVIHALKOVA, Radka ZOUNKOVÁ and Luděk BLÁHA. The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria. \textit{Toxicon}. OXFORD: Elsevier Science, 2013, vol.~76, No~2013, p.~187-196. ISSN~0041-0101. Available from: https://dx.doi.org/10.1016/j.toxicon.2013.10.011.

Detailed Information on Publication Record