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@proceedings{1161683, author = {Pekarčíková, Lucie and Knopfová, Lucia and Beneš, Petr and Hermanová, Markéta and Tichý, Michal and Šmarda, Jan}, booktitle = {Korean Journal of Pathology}, title = {The c-Myb-induced resistance of colon cancer cells to cisplatin is mediated by the p38 MAP kinase}, year = {2013} }
TY - CONF ID - 1161683 AU - Pekarčíková, Lucie - Knopfová, Lucia - Beneš, Petr - Hermanová, Markéta - Tichý, Michal - Šmarda, Jan PY - 2013 TI - The c-Myb-induced resistance of colon cancer cells to cisplatin is mediated by the p38 MAP kinase N2 - Background: The c-Myb transcription factor is essential for maintenance of stem-progenitor cells in colon epithelia and its expression is deregulated in premalignant adenomatous polyps and colorectal carcinomas. Patients with colorectal carcinoma exhibiting upregulated c- Myb expression have poor prognosis. This can result from low sensitivity of cancer cells to cytotoxic agents. Methods: In our study, increased resistance of colon carcinoma CT26 and HCT116 cells overproducing exogenous c-Myb to cytotoxic agents was observed. These cells lost sensitivity to cisplatin and doxorubicin upon upregulation of c-Myb. The effect of c-Myb can be mediated by the mitogen-activated protein kinase (MAPK) signaling since phosphorylation of the MAPKs p38 and JNK was enhanced in cells exhibiting high expression of exogenous c-Myb. Results: To determine functional contribution of these kinases to resistance of the c-Myb-overexpressing cells to cisplatin, we used BIRB796 to inhibit activity of p38 and SP600125 to inhibit JNK. We found that the c-Myb-induced resistance of CT26 cells to cisplatin can be reversed by BIRB796. Conclusions: This result documents that it is the p38 kinase that mediates the effects of c-Myb on sensitivity of colon cancer CT26 cells to cisplatin (This study was supported by grant NT13441/2012 of the Internal Grant Agency of the Ministry of Health, Czech Republic). ER -
PEKARČÍKOVÁ, Lucie, Lucia KNOPFOVÁ, Petr BENEŠ, Markéta HERMANOVÁ, Michal TICHÝ and Jan ŠMARDA. The c-Myb-induced resistance of colon cancer cells to cisplatin is mediated by the p38 MAP kinase. In \textit{Korean Journal of Pathology}. 2013. ISSN~1738-1843.
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