Elucidation of signaling molecules involved in ergosterol
perception in tobacco

J 2013

Elucidation of signaling molecules involved in ergosterol perception in tobacco

DADÁKOVÁ, Kateřina, Jitka KLEMPOVÁ, Tereza JENDRIŠÁKOVÁ, Jan LOCHMAN, Tomáš KAŠPAROVSKÝ et. al.

Basic information

Original name

Elucidation of signaling molecules involved in ergosterol perception in tobacco

Authors

DADÁKOVÁ, Kateřina (203 Czech Republic, belonging to the institution), Jitka KLEMPOVÁ (203 Czech Republic, belonging to the institution), Tereza JENDRIŠÁKOVÁ (203 Czech Republic), Jan LOCHMAN (203 Czech Republic, belonging to the institution) and Tomáš KAŠPAROVSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant Physiol Biochem, Elsevier Science, 2013, 0981-9428

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.352

RIV identification code

RIV/00216224:14310/13:00066829

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.plaphy.2013.09.009

UT WoS

000329007000016

Keywords in English

Ergosterol; Tobacco; Defense; Signaling; Salicylic acid; Jasmonic acid; Spermine

Abstract

V originále

Ergosterol, a principal compound of the fungal plasma membrane, is regarded as a pathogen-associated molecular pattern. In the present study, the role of salicylic acid (SA), jasmonic acid (JA) and spermine signaling pathways after ergosterol elicitation were evaluated. SA, JA and spermine production, as well as accumulation of transcripts for a lipoxygenase (NaLOX3) gene, the phenylalanine-ammonia lyase gene, selected pathogenesis-related genes (PR1, PR5), and peroxidase tPOXC1 were determined in tobacco (Nicotiana tabacum L. cv. Xanthi) in response to ergosterol elicitation. To understand the sequence of the signaling cascade, several representative steps involved in the synthesis of crucial signaling molecules were targeted using specific inhibitors. SA signaling pathway, together with calmodulin-dependent protein kinases and nitric oxide, was demonstrated to play an important role in the induction of defense-related genes following ergosterol treatment. The results suggested that nitric oxide participates in defense-related gene activation following ergosterol treatment but does not directly participate in activation of reactive oxygen species production. The induction of PR5 and tPOXC1 transcripts was found to be not fully dependent on calmodulin/Ca2+ and SA signaling, contrary to the PR1a transcript. A possible candidate for this SA-independent pathway is the spermine pathway, as elevated spermine levels were detected following ergosterol treatment.

Links

GAP501/12/0590, research and development project

Name: Charakterizace procesů zapojených v indukci resistence rostlin na patogeny s využitím elicitinů se změněnou schopností vyvolávat obranné reakce

Investor: Czech Science Foundation

Citovat

DADÁKOVÁ, Kateřina, Jitka KLEMPOVÁ, Tereza JENDRIŠÁKOVÁ, Jan LOCHMAN and Tomáš KAŠPAROVSKÝ. Elucidation of signaling molecules involved in ergosterol perception in tobacco. Plant Physiol Biochem. Elsevier Science, 2013, vol. 73, december, p. 121-127. ISSN 0981-9428. Available from: https://dx.doi.org/10.1016/j.plaphy.2013.09.009.

@article{1162048,
   author = {Dadáková, Kateřina and Klempová, Jitka and Jendrišáková, Tereza and Lochman, Jan and Kašparovský, Tomáš},
   article_number = {december},
   doi = {http://dx.doi.org/10.1016/j.plaphy.2013.09.009},
   keywords = {Ergosterol; Tobacco; Defense; Signaling; Salicylic acid; Jasmonic acid; Spermine},
   language = {eng},
   issn = {0981-9428},
   journal = {Plant Physiol Biochem},
   title = {Elucidation of signaling molecules involved in ergosterol perception in tobacco},
   url = {http://www.sciencedirect.com/science/article/pii/S0981942813003252},
   volume = {73},
   year = {2013}
}

TY  - JOUR
ID  - 1162048
AU  - Dadáková, Kateřina - Klempová, Jitka - Jendrišáková, Tereza - Lochman, Jan - Kašparovský, Tomáš
PY  - 2013
TI  - Elucidation of signaling molecules involved in ergosterol perception in tobacco
JF  - Plant Physiol Biochem
VL  - 73
IS  - december
SP  - 121-127
EP  - 121-127
PB  - Elsevier Science
SN  - 09819428
KW  - Ergosterol
KW  - Tobacco
KW  - Defense
KW  - Signaling
KW  - Salicylic acid
KW  - Jasmonic acid
KW  - Spermine
UR  - http://www.sciencedirect.com/science/article/pii/S0981942813003252
L2  - http://www.sciencedirect.com/science/article/pii/S0981942813003252
N2  - Ergosterol, a principal compound of the fungal plasma membrane, is regarded as a pathogen-associated molecular pattern. In the present study, the role of salicylic acid (SA), jasmonic acid (JA) and spermine signaling pathways after ergosterol elicitation were evaluated. SA, JA and spermine production, as well as accumulation of transcripts for a lipoxygenase (NaLOX3) gene, the phenylalanine-ammonia lyase gene, selected pathogenesis-related genes (PR1, PR5), and peroxidase tPOXC1 were determined in tobacco (Nicotiana tabacum L. cv. Xanthi) in response to ergosterol elicitation. To understand the sequence of the signaling cascade, several representative steps involved in the synthesis of crucial signaling molecules were targeted using specific inhibitors. SA signaling pathway, together with calmodulin-dependent protein kinases and nitric oxide, was demonstrated to play an important role in the induction of defense-related genes following ergosterol treatment. The results suggested that nitric oxide participates in defense-related gene activation following ergosterol treatment but does not directly participate in activation of reactive oxygen species production. The induction of PR5 and tPOXC1 transcripts was found to be not fully dependent on calmodulin/Ca2+ and SA signaling, contrary to the PR1a transcript. A possible candidate for this SA-independent pathway is the spermine pathway, as elevated spermine levels were detected following ergosterol treatment.
ER  -

DADÁKOVÁ, Kateřina, Jitka KLEMPOVÁ, Tereza JENDRIŠÁKOVÁ, Jan LOCHMAN and Tomáš KAŠPAROVSKÝ. Elucidation of signaling molecules involved in ergosterol perception in tobacco. \textit{Plant Physiol Biochem}. Elsevier Science, 2013, vol.~73, december, p.~121-127. ISSN~0981-9428. Available from: https://dx.doi.org/10.1016/j.plaphy.2013.09.009.

Detailed Information on Publication Record