Mother and child T cell receptor repertoires: deep profiling study

@article{1163562,
   author = {Putintseva, E. and Britanova, Olga and Staroverov, D. and Merzlyak, E. and Turchaninova, M. and Shugay, M. and Bolotin, D. and Pogorelyy, M. and Mamedov, I. and Bobrynina, V. and Maschan, M. and Lebedev, Y. and Chudakov, Dmitriy},
   article_location = {Lausanne},
   article_number = {Dec},
   doi = {http://dx.doi.org/10.3389/fimmu.2013.00463},
   keywords = {TCR repertoires; NGS; maternal fetal exchange; public clonotypes; T cell receptor; haploidentical transplantation; autoimmune diseases; microchimerism},
   language = {eng},
   issn = {1664-3224},
   journal = {Frontiers in Immunology},
   title = {Mother and child T cell receptor repertoires: deep profiling study},
   url = {http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00463/full},
   volume = {4},
   year = {2013}
}

TY  - JOUR
ID  - 1163562
AU  - Putintseva, E. - Britanova, Olga - Staroverov, D. - Merzlyak, E. - Turchaninova, M. - Shugay, M. - Bolotin, D. - Pogorelyy, M. - Mamedov, I. - Bobrynina, V. - Maschan, M. - Lebedev, Y. - Chudakov, Dmitriy
PY  - 2013
TI  - Mother and child T cell receptor repertoires: deep profiling study
JF  - Frontiers in Immunology
VL  - 4
IS  - Dec
SP  - nestránkováno
EP  - nestránkováno
PB  - Frontiers Research Foundation
SN  - 16643224
KW  - TCR repertoires
KW  - NGS
KW  - maternal fetal exchange
KW  - public clonotypes
KW  - T cell receptor
KW  - haploidentical transplantation
KW  - autoimmune diseases
KW  - microchimerism
UR  - http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00463/full
L2  - http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00463/full
N2  - The relationship between maternal and child immunity has been actively studied in the context of complications during pregnancy, autoimmune diseases, and haploidentical transplantation of hematopoietic stem cells and solid organs. Here, we have for the first time used high throughput Illumina HiSeq sequencing to perform deep quantitative profiling of T cell receptor (TCR) repertoires for peripheral blood samples of three mothers and their six children. Advanced technology allowed accurate identification of 5 105 to 2 106 TCR beta clonotypes per individual. We performed comparative analysis of these TCR repertoires with the aim of revealing characteristic features that distinguish related mother-child pairs, such as relative TCR beta variable segment usage frequency and relative overlap of TCR beta complementarity-determining region 3 (CDR3) repertoires. We show that thymic selection essentially and similarly shapes the initial output of the TCR recombination machinery in both related and unrelated pairs, with minor effect from inherited differences. The achieved depth of TCR profiling also allowed us to test the hypothesis that mature T cells transferred across the placenta during pregnancy can expand and persist as functional microchimeric clones in their new host, using characteristic TCR beta CDR3 variants as clonal identifiers.
ER  - 

PUTINTSEVA, E., Olga BRITANOVA, D. STAROVEROV, E. MERZLYAK, M. TURCHANINOVA, M. SHUGAY, D. BOLOTIN, M. POGORELYY, I. MAMEDOV, V. BOBRYNINA, M. MASCHAN, Y. LEBEDEV a Dmitriy CHUDAKOV. Mother and child T cell receptor repertoires: deep profiling study. \textit{Frontiers in Immunology}. Lausanne: Frontiers Research Foundation, 2013, roč.~4, Dec, s.~nestránkováno, 13 s. ISSN~1664-3224. doi:10.3389/fimmu.2013.00463.