J 2014

MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics

TOMALOVÁ, Iva, Pavla FOLTYNOVÁ, Viktor KANICKÝ and Jan PREISLER

Basic information

Original name

MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics

Authors

TOMALOVÁ, Iva (203 Czech Republic, belonging to the institution), Pavla FOLTYNOVÁ (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution)

Edition

Analytical Chemistry, Washington, American Chemical Society, 2014, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.636

RIV identification code

RIV/00216224:14740/14:00073485

Organization unit

Central European Institute of Technology

UT WoS

000329548700068

Keywords in English

MALDI MS; ICP MS; single run; capillary electrophoresis

Tags

International impact, Reviewed
Změněno: 15/2/2018 22:11, prof. Mgr. Jan Preisler, Ph.D.

Abstract

V originále

In this work, a novel approach based on off-line coupling of a single run of capillary electrophoresis (CE) separation to both matrix-assisted laser desorption/ionization (MALDI) and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS) is presented. Using a liquid junction and subatmospheric deposition chamber, CE fractions were extracted from a separation capillary and collected as 20-nL droplets on a custom-built polyethylene terephthalate glycol (PETG) target plate coated with a 10-nm gold layer which guaranteed compatibility with both MALDI and SALD ICP techniques. The MALDI matrix solution was then added to the produced spots. After it was dried, the separation record was consecutively analyzed in MALDI MS and ICP MS instruments. Thus, both proteomic and metallomic information was obtained off-line from a single CE run. The concept was demonstrated by the analysis of a mixture of rabbit liver metallothionein isoforms. In an additional study, the droplets representing the archived separation record were alternately mixed with two different MALDI matrices to obtain complementary information on both the apoproteins and their complexes with metals from a single separation run. The presented technique is a viable alternative to online coupling of column separation to electrospray MS and nebulizer ICP MS.

Links

ED1.1.00/02.0068, research and development project
Name: CEITEC - central european institute of technology
EE2.3.30.0009, research and development project
Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci
GAP206/12/0538, research and development project
Name: Nové strategie úpravy vzorků pro desorpční hmotnostní spektrometrii
Investor: Czech Science Foundation