Detailed Information on Publication Record
2014
MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics
TOMALOVÁ, Iva, Pavla FOLTYNOVÁ, Viktor KANICKÝ and Jan PREISLERBasic information
Original name
MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics
Authors
TOMALOVÁ, Iva (203 Czech Republic, belonging to the institution), Pavla FOLTYNOVÁ (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution)
Edition
Analytical Chemistry, Washington, American Chemical Society, 2014, 0003-2700
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.636
RIV identification code
RIV/00216224:14740/14:00073485
Organization unit
Central European Institute of Technology
UT WoS
000329548700068
Keywords in English
MALDI MS; ICP MS; single run; capillary electrophoresis
Tags
Tags
International impact, Reviewed
Změněno: 15/2/2018 22:11, prof. Mgr. Jan Preisler, Ph.D.
Abstract
V originále
In this work, a novel approach based on off-line coupling of a single run of capillary electrophoresis (CE) separation to both matrix-assisted laser desorption/ionization (MALDI) and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS) is presented. Using a liquid junction and subatmospheric deposition chamber, CE fractions were extracted from a separation capillary and collected as 20-nL droplets on a custom-built polyethylene terephthalate glycol (PETG) target plate coated with a 10-nm gold layer which guaranteed compatibility with both MALDI and SALD ICP techniques. The MALDI matrix solution was then added to the produced spots. After it was dried, the separation record was consecutively analyzed in MALDI MS and ICP MS instruments. Thus, both proteomic and metallomic information was obtained off-line from a single CE run. The concept was demonstrated by the analysis of a mixture of rabbit liver metallothionein isoforms. In an additional study, the droplets representing the archived separation record were alternately mixed with two different MALDI matrices to obtain complementary information on both the apoproteins and their complexes with metals from a single separation run. The presented technique is a viable alternative to online coupling of column separation to electrospray MS and nebulizer ICP MS.
Links
ED1.1.00/02.0068, research and development project |
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EE2.3.30.0009, research and development project |
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GAP206/12/0538, research and development project |
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