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@article{1163964,
author = {Tomalová, Iva and Foltynová, Pavla and Kanický, Viktor and Preisler, Jan},
article_location = {Washington},
article_number = {1},
doi = {http://dx.doi.org/10.1021/ac402941e},
keywords = {MALDI MS; ICP MS; single run; capillary electrophoresis},
language = {eng},
issn = {0003-2700},
journal = {Analytical Chemistry},
title = {MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics},
url = {http://pubs.acs.org/doi/abs/10.1021/ac402941e},
volume = {86},
year = {2014}
}
TY - JOUR
ID - 1163964
AU - Tomalová, Iva - Foltynová, Pavla - Kanický, Viktor - Preisler, Jan
PY - 2014
TI - MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics
JF - Analytical Chemistry
VL - 86
IS - 1
SP - 647-654
EP - 647-654
PB - American Chemical Society
SN - 00032700
KW - MALDI MS
KW - ICP MS
KW - single run
KW - capillary electrophoresis
UR - http://pubs.acs.org/doi/abs/10.1021/ac402941e
L2 - http://pubs.acs.org/doi/abs/10.1021/ac402941e
N2 - In this work, a novel approach based on off-line coupling of a single run of capillary electrophoresis (CE) separation to both matrix-assisted laser desorption/ionization (MALDI) and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS) is presented. Using a liquid junction and subatmospheric deposition chamber, CE fractions were extracted from a separation capillary and collected as 20-nL droplets on a custom-built polyethylene terephthalate glycol (PETG) target plate coated with a 10-nm gold layer which guaranteed compatibility with both MALDI and SALD ICP techniques. The MALDI matrix solution was then added to the produced spots. After it was dried, the separation record was consecutively analyzed in MALDI MS and ICP MS instruments. Thus, both proteomic and metallomic information was obtained off-line from a single CE run. The concept was demonstrated by the analysis of a mixture of rabbit liver metallothionein isoforms. In an additional study, the droplets representing the archived separation record were alternately mixed with two different MALDI matrices to obtain complementary information on both the apoproteins and their complexes with metals from a single separation run. The presented technique is a viable alternative to online coupling of column separation to electrospray MS and nebulizer ICP MS.
ER -
TOMALOVÁ, Iva, Pavla FOLTYNOVÁ, Viktor KANICKÝ and Jan PREISLER. MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics. \textit{Analytical Chemistry}. Washington: American Chemical Society, vol.~86, No~1, p.~647-654. ISSN~0003-2700. doi:10.1021/ac402941e. 2014.
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