Life cycle of Cryptosporidium muris in two rodents with
different responses to parasitization

J 2014

Life cycle of Cryptosporidium muris in two rodents with different responses to parasitization

MELICHEROVÁ, Janka, Jana ILGOVÁ, Martin KVÁČ, Bohumil SAK, Břetislav KOUDELA et. al.

Basic information

Original name

Life cycle of Cryptosporidium muris in two rodents with different responses to parasitization

Authors

MELICHEROVÁ, Janka (703 Slovakia, guarantor, belonging to the institution), Jana ILGOVÁ (703 Slovakia, belonging to the institution), Martin KVÁČ (203 Czech Republic), Bohumil SAK (203 Czech Republic), Břetislav KOUDELA (203 Czech Republic) and Andrea VALIGUROVÁ (703 Slovakia, belonging to the institution)

Edition

Parasitology, Cambridge University Press, 2014, 0031-1820

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.560

RIV identification code

RIV/00216224:14310/14:00073486

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1017/S0031182013001637

UT WoS

000331903700013

Keywords (in Czech)

cryptosporidia development gastric oocyst pathology Type II merogony

Keywords in English

cryptosporidia development gastric oocyst pathology Type II merogony

Abstract

V originále

This study focuses on mapping the life cycle of Cryptosporidium muris in two laboratory rodents; BALB/c mice and the southern multimammate rat Mastomys coucha, differing in their prepatent and patent periods. Both rodents were simultaneously experimentally inoculated with viable oocysts of C. muris (strain TS03). Animals were dissected and screened for the presence of the parasite using a combined morphological approach and nested PCR (SSU rRNA) at different times after inoculation. The occurrence of first developmental stages of C. muris in stomach was detected at 2.5 DPI. The presence of Type II merogony, appearing 36 hours later than Type I merogony, was confirmed in both rodents. Oocysts exhibiting different size and thickness of their wall were observed from 5 DPI onwards in stomach of both host models. The early phase of parasitisation in BALB/c mice progressed rapidly, with a prepatent period of 7.5–10 days; whereas in M. coucha, the developmental stages of C. muris were first observed 12 hours later in comparison with BALB/c mice and prepatent period was longer (18–21 days). Similarly, the patent periods of BALB/c mice and M. coucha differed considerably, i.e. 10–15 days versus chronic infection throughout the life of the host, respectively.

Links

GAP505/11/1163, research and development project

Name: Protizánětlivá aktivita extraktů z rostlin Indonésie a jejich účinek na průběh oportunních parazitóz

Investor: Czech Science Foundation

GPP506/10/P372, research and development project

Name: Srovnávací morfologie a imunohistochemie v hodnocení fylogenetických vztahů mezi zástupci raných linií kmene Apicomplexa

Investor: Czech Science Foundation

Citovat

MELICHEROVÁ, Janka, Jana ILGOVÁ, Martin KVÁČ, Bohumil SAK, Břetislav KOUDELA and Andrea VALIGUROVÁ. Life cycle of Cryptosporidium muris in two rodents with different responses to parasitization. Parasitology. Cambridge University Press, 2014, vol. 141, No 2, p. 287-303. ISSN 0031-1820. Available from: https://dx.doi.org/10.1017/S0031182013001637.

@article{1163993,
   author = {Melicherová, Janka and Ilgová, Jana and Kváč, Martin and Sak, Bohumil and Koudela, Břetislav and Valigurová, Andrea},
   article_number = {2},
   doi = {http://dx.doi.org/10.1017/S0031182013001637},
   keywords = {cryptosporidia development gastric oocyst pathology Type II merogony},
   language = {eng},
   issn = {0031-1820},
   journal = {Parasitology},
   title = {Life cycle of Cryptosporidium muris in two rodents with different responses to parasitization},
   url = {http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=9041945},
   volume = {141},
   year = {2014}
}

TY  - JOUR
ID  - 1163993
AU  - Melicherová, Janka - Ilgová, Jana - Kváč, Martin - Sak, Bohumil - Koudela, Břetislav - Valigurová, Andrea
PY  - 2014
TI  - Life cycle of Cryptosporidium muris in two rodents with different responses to parasitization
JF  - Parasitology
VL  - 141
IS  - 2
SP  - 287-303
EP  - 287-303
PB  - Cambridge University Press
SN  - 00311820
KW  - cryptosporidia development gastric oocyst pathology Type II merogony
UR  - http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=9041945
N2  - This study focuses on mapping the life cycle of Cryptosporidium muris in two laboratory rodents; BALB/c mice and the southern multimammate rat Mastomys coucha, differing in their prepatent and patent periods. Both rodents were simultaneously experimentally inoculated with viable oocysts of C. muris (strain TS03). Animals were dissected and screened for the presence of the parasite using a combined morphological approach and nested PCR (SSU rRNA) at different times after inoculation. The occurrence of first developmental stages of C. muris in stomach was detected at 2.5 DPI. The presence of Type II merogony, appearing 36 hours later than Type I merogony, was confirmed in both rodents. Oocysts exhibiting different size and thickness of their wall were observed from 5 DPI onwards in stomach of both host models. The early phase of parasitisation in BALB/c mice progressed rapidly, with a prepatent period of 7.5–10 days; whereas in M. coucha, the developmental stages of C. muris were first observed 12 hours later in comparison with BALB/c mice and prepatent period was longer (18–21 days). Similarly, the patent periods of BALB/c mice and M. coucha differed considerably, i.e. 10–15 days versus chronic infection throughout the life of the host, respectively.
ER  -

MELICHEROVÁ, Janka, Jana ILGOVÁ, Martin KVÁČ, Bohumil SAK, Břetislav KOUDELA and Andrea VALIGUROVÁ. Life cycle of Cryptosporidium muris in two rodents with different responses to parasitization. \textit{Parasitology}. Cambridge University Press, 2014, vol.~141, No~2, p.~287-303. ISSN~0031-1820. Available from: https://dx.doi.org/10.1017/S0031182013001637.

Detailed Information on Publication Record