CAPILLARY ELECTROPHORETIC SCREENING OF TOXIC METABOLITES IN VARIOUS BODY FLUIDS - A SIMPLE DIAGNOSTIC TOOL TO DIFFERENTIATE METHANOL AND ETHYLENE GLYCOL POISONING

ĎURČ, Pavol, Petr KUBÁŇ, Miroslava BITTOVÁ and František FORET. CAPILLARY ELECTROPHORETIC SCREENING OF TOXIC METABOLITES IN VARIOUS BODY FLUIDS - A SIMPLE DIAGNOSTIC TOOL TO DIFFERENTIATE METHANOL AND ETHYLENE GLYCOL POISONING. Online. In František Foret, Jana Křenková, Andras Guttman, Karel Klepárník, Petr Boček. Chemické listy. Vol. 107. Praha: Česká společnost chemická, 2013, p. "S355"-"S357", 3 pp. ISSN 1213-7103.

Basic information

• Original name: CAPILLARY ELECTROPHORETIC SCREENING OF TOXIC METABOLITES IN VARIOUS BODY FLUIDS - A SIMPLE DIAGNOSTIC TOOL TO DIFFERENTIATE METHANOL AND ETHYLENE GLYCOL POISONING
• Authors: ĎURČ, Pavol (703 Slovakia, belonging to the institution), Petr KUBÁŇ (203 Czech Republic, guarantor, belonging to the institution), Miroslava BITTOVÁ (203 Czech Republic, belonging to the institution) and František FORET (203 Czech Republic, belonging to the institution).
• Edition: Vol. 107. Praha, Chemické listy, p. "S355"-"S357", 3 pp. 2013.
• Publisher: Česká společnost chemická

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Publication form: electronic version available online
• WWW: Chemické listy
• Impact factor: Impact factor: 0.196
• RIV identification code: RIV/00216224:14740/13:00066859
• Organization unit: Central European Institute of Technology
• ISSN: 1213-7103
• UT WoS: 000328730800030
• Keywords (in Czech): kapilární elektroforéza; bezkontaktní konduktometrická detekce; metanol; ethylenglykol; toxické metabolity
• Keywords in English: capillary electrophoresis; contactless conductivity detection; methanol; ethylenglycol; toxic metabolites
• Tags: rivok

Abstract

A new, simple and rapid capillary electrophoretic method with contactless conductivity detection for screening of formate, oxalate and glycolate in various body samples (blood serum, saliva, urine, exhaled breath condensate) is presented. The target analytes are separated in less than 6 minutes in an electrolyte composed of 50 mM L-histidine and 50 mM 2-(N-morpholino) ethanesulfonic acid at pH 5.9. A short (33 cm) fused silica capillary with 25 um ID is used. LODs range from 0.4 to 1.25 uM. The method provides a simple and rapid diagnostic test in suspected intoxication of industrial chemicals and is able to distinguish the ingested liquid, based on its metabolite trace providing a fast screening tool that can be applicable in clinical practice.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• GA13-21919S, research and development project: Name: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.

Investor: Czech Science Foundation