Rapid Matrix-Assisted Laser Desorption/Ionization
Time-of-Flight Mass Spectrometry Imaging with Scanning
Desorption Laser Beam

J 2014

Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam

BEDNAŘÍK, Antonín; Pavel KUBA; Eugene MOSKOVETS; Iva TOMALOVÁ; Pavel KRÁSENSKÝ et. al.

Basic information

Original name

Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam

Authors

BEDNAŘÍK, Antonín (203 Czech Republic, belonging to the institution); Pavel KUBA (203 Czech Republic); Eugene MOSKOVETS (643 Russian Federation); Iva TOMALOVÁ (203 Czech Republic, belonging to the institution); Pavel KRÁSENSKÝ (203 Czech Republic, belonging to the institution); Pavel HOUŠKA (203 Czech Republic) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution)

Edition

Analytical Chemistry, Washington, American Chemical Society, 2014, 0003-2700

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 5.636

RIV identification code

RIV/00216224:14740/14:00073489

Organization unit

Central European Institute of Technology

DOI

https://doi.org/10.1021/ac402823n

UT WoS

000330204500004

EID Scopus

2-s2.0-84892737845

Keywords in English

Mass spectrometry imaging; laser beam scanning; MALDI; TOF; high-throughput

Abstract

In the original language

This work shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in 11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP206/12/0538, research and development project

Name: Nové strategie úpravy vzorků pro desorpční hmotnostní spektrometrii

Investor: Czech Science Foundation

GCP206/10/J012, research and development project

Name: Analýza biomolekul hmotnostní spektrometrií s laserovou desorpcí/ionizací za účasti nanomateriálů

Investor: Czech Science Foundation

Cite

BEDNAŘÍK, Antonín; Pavel KUBA; Eugene MOSKOVETS; Iva TOMALOVÁ; Pavel KRÁSENSKÝ; Pavel HOUŠKA and Jan PREISLER. Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam. Analytical Chemistry. Washington: American Chemical Society, 2014, vol. 86, No 2, p. 982-986. ISSN 0003-2700. Available from: https://doi.org/10.1021/ac402823n.

@article{1164080,
   author = {Bednařík, Antonín and Kuba, Pavel and Moskovets, Eugene and Tomalová, Iva and Krásenský, Pavel and Houška, Pavel and Preisler, Jan},
   article_location = {Washington},
   article_number = {2},
   doi = {https://doi.org/10.1021/ac402823n},
   keywords = {Mass spectrometry imaging; laser beam scanning; MALDI; TOF; high-throughput},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam},
   url = {http://pubs.acs.org/doi/abs/10.1021/ac402823n},
   volume = {86},
   year = {2014}
}

TY  - JOUR
ID  - 1164080
AU  - Bednařík, Antonín - Kuba, Pavel - Moskovets, Eugene - Tomalová, Iva - Krásenský, Pavel - Houška, Pavel - Preisler, Jan
PY  - 2014
TI  - Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam
JF  - Analytical Chemistry
VL  - 86
IS  - 2
SP  - 982-986
EP  - 982-986
PB  - American Chemical Society
SN  - 00032700
KW  - Mass spectrometry imaging
KW  - laser beam scanning
KW  - MALDI
KW  - TOF
KW  - high-throughput
UR  - http://pubs.acs.org/doi/abs/10.1021/ac402823n
L2  - http://pubs.acs.org/doi/abs/10.1021/ac402823n
N2  - This work shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in 11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.
ER  -

BEDNAŘÍK, Antonín; Pavel KUBA; Eugene MOSKOVETS; Iva TOMALOVÁ; Pavel KRÁSENSKÝ; Pavel HOUŠKA and Jan PREISLER. Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam. \textit{Analytical Chemistry}. Washington: American Chemical Society, 2014, vol.~86, No~2, p.~982-986. ISSN~0003-2700. Available from: https://doi.org/10.1021/ac402823n.

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